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基于81°倾斜光纤光栅的新城疫病毒免疫传感器

Newcastle Disease Virus Immunosensor Based on 81° Tilted Fiber Grating

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摘要

提出一种基于81°倾斜光纤光栅(81°-TFG)的新城疫病毒(NDV)免疫传感器。分析81°-TFG传感器的基本原理和传感特性。利用金黄色葡萄球菌蛋白A(SPA)修饰81°-TFG的表面,然后将自制的高纯度新城疫病毒单克隆抗体(MAbs)通过SPA分子固定于81°-TFG表面,从而制成对NDV抗原特异性检测的传感器。实验结果表明:该免疫传感器对NDV的探测极限在0.1 ~0.2 ng/mL之间,检测饱和点约为1.0 ng/mL;在0~1.0 ng/mL范围内具有良好的线性度(R2约为 0.982),灵敏度约为342 pm/(ng·mL-1);且该传感器具有良好的可重用性和对NDV抗原的高度特异性,并能用于NDV的临床检测。相对于传统的免疫荧光技术、酶联免疫等生化检测方法,本文提出的方法具有免标记、操作简便、快速检测等优点。

Abstract

A type of newcastle disease virus (NDV) immunosensor based on 81° tilted fiber grating (81°-TFG) is proposed. The basic principle and sensing properties of the 81°-TFG are briefly analyzed. Staphylococcus aureus protein A (SPA) is used to modify the surface of 81°-TFG, and then highly purified NDV monoclonal antibodies (MAbs) are immobilized on 81°-TFG surface through SPA molecules. Finally, the NDV immunosensor is successfully developed. The experimental results show that the immunosensor has a lowest limit of detection to NDV of between 0.1 ng/mL and 0.2 ng/mL, gets saturated at about 1.0 ng/mL, and has good linearity with R2 of about 0.982 and sensitivity of about 342 pm/(ng·mL-1) in the concentration range from 0 to 1.0 ng/mL. In addition, this immunosensor shows good reusability and high specificity to NDV, and can also be used in clinical test for NDV. Compared with the traditional biochemical methods such as immunofluorescence technique, enzyme-linked immunoabsorbent assay, this technique proposed in this paper has the advantages of no mark, simple operation, fast detection.

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中图分类号:TN253

DOI:10.3788/AOS201737.1106001

所属栏目:光纤光学与光通信

基金项目:国家自然科学基金(61505017, 61327004, 61421002)、中国博士后基金(2016M592649)、重庆市科委基础与前沿研究项目(cstc2014jcyjA0081, cstc2015jcyjA40035)、重庆市高等学校青年骨干教师资助计划、重庆市教委科学技术研究项目(KJ1709192,KJ1709211)、重庆高校创新团队建设计划(CXTDX201601031)、重庆理工大学研究生创新基金(YCX2016103)

收稿日期:2017-03-28

修改稿日期:2017-06-16

网络出版日期:--

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罗彬彬:重庆理工大学光纤传感与光电检测重庆市重点实验室, 重庆 400054电子科技大学光电信息学院, 四川 成都 610054
吴胜昔:重庆理工大学药物化学与分子药理学重庆市重点实验室, 重庆 400054
王玲玲:重庆理工大学药物化学与分子药理学重庆市重点实验室, 重庆 400054
张中豪:重庆理工大学药物化学与分子药理学重庆市重点实验室, 重庆 400054
徐杨非:重庆理工大学光纤传感与光电检测重庆市重点实验室, 重庆 400054
蒋棚俊:重庆理工大学药物化学与分子药理学重庆市重点实验室, 重庆 400054
石胜辉:重庆理工大学光纤传感与光电检测重庆市重点实验室, 重庆 400054
邹雪:重庆理工大学光纤传感与光电检测重庆市重点实验室, 重庆 400054
鲁姣:重庆理工大学光纤传感与光电检测重庆市重点实验室, 重庆 400054
赵明富:重庆理工大学光纤传感与光电检测重庆市重点实验室, 重庆 400054
刘永:电子科技大学光电信息学院, 四川 成都 610054

联系人作者:吴胜昔(sxwu2004@cqut.edu.cn)

备注:罗彬彬(1981-),男,博士,副教授,主要从事光纤传感器技术方面的研究。

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引用该论文

Luo Binbin,Wu Shengxi,Wang Lingling,Zhang Zhonghao,Xu Yangfei,Jiang Pengjun,Shi Shenghui,Zou Xue,Lu Jiao,Zhao Mingfu,Liu Yong. Newcastle Disease Virus Immunosensor Based on 81° Tilted Fiber Grating[J]. Acta Optica Sinica, 2017, 37(11): 1106001

罗彬彬,吴胜昔,王玲玲,张中豪,徐杨非,蒋棚俊,石胜辉,邹雪,鲁姣,赵明富,刘永. 基于81°倾斜光纤光栅的新城疫病毒免疫传感器[J]. 光学学报, 2017, 37(11): 1106001

被引情况

【1】薛渊泽,王学锋,罗明明,唐才杰,蓝天. 再生光纤布拉格光栅的研究进展. 激光与光电子学进展, 2018, 55(2): 20007--1

【2】郭艳城,刘艳格,王志,姚卓坤. 少模光纤长周期光栅双峰谐振及双参量传感. 光学学报, 2018, 38(9): 906003--1

【3】罗彬彬,赵明富,石胜辉,刘 永. 基于星形金纳米颗粒修饰的81°倾斜光纤光栅生物传感器. 半导体光电, 2018, 39(5): 634-639

【4】石胜辉,吴德操,王鑫,聂青林,刘志江,罗彬彬,刘恩华,柳鹏,赵明富. 氧化石墨烯包覆金纳米壳修饰长周期光栅的免疫传感器. 光学学报, 2020, 40(18): 1806001--1

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