目的: 探讨烧伤膏对烫伤小鼠皮肤创面修复的可能作用机制。方法: 将50只小鼠随机分为5组: 正常组、模型组, 烧伤膏高、中、低剂量组。除正常组和模型组外, 烧伤膏实验组小鼠每天外涂烧伤膏治疗, 连用15天。治疗结束后, 采用Masson染色技术检测各组小鼠皮肤厚度及细胞病理形态; 采用生化法检测皮肤中羟脯氨酸(Hyp)含量; 采用ELISA技术检测皮肤中的TNF-α, IL-1β、Collagen I、EGF和TGF-β1的含量。结果: 与正常组相比, 模型组小鼠皮肤烫伤创面可见表皮细胞脱落, 结构模糊, 细胞变性坏死, 表皮和真皮厚度明显减少, 胶原纤维变薄, 排列紊乱无序, 呈断裂状; 烧伤后创面局部组织中Hyp、TNF-α、IL-1β的含量显著升高(P<0.01, P<0.01, P<0.01), 而EGF、TGF-β1和Collagen I含量均明显减少(P<0.01, P<0.01, P<0.01), 而应用烧伤膏治疗15天后, 上述各项实验指标明显得到改善。结论: 烧伤膏抑制创面早期感染, 减少胶原蛋白的分解和促进后期创面修复的作用可能与抑制炎性细胞因子和增加皮肤相关生长因子表达的作用机制有关。

Objective: To investigate the possible mechanism of effects of Burn Ointment on burn wound healing of the skin tissue in scalded model mice. Methods: Fifty mice were divided into 5 groups randomly: control group, model group, high dose group, middle dose group and low dose group of Burn Ointment. The experimental groups were applied by Burn Ointment for 15 days except the control group and model group. After treatment, skin thickness and cell pathomorphology were detected by Masson staining. Moreover, content of hydroxyproline (Hyp) was measured by biochemical method. Contents of TNF-α, IL-1β, Collagen I,EGF and TGF-β1 were detected by ELISA. Results: Compared with control group, from the observation of the scalded wound surface of mice, we found that structure of skin was unclear and epidermal cells were detached. Cells appeared degeneration and necrosis. The thickness of the epidermis and dermis was reduced. Collagen fibers were thinner, disordered and fractured. The contents of Hyp, TNF- and IL-1β of the model group were all significantly higher than those of control group (P all<0.01), while the contents of EGF, TGF-β1 and Collagen I were decreased markedly (P all<0.01). After Burn Ointment treatment, every indicator of experiment was significantly improved. Conclusion: The Burn Ointment can significantly inhibit early wound infection, reduce breakdown of collagen and promote wound healing, which possibly related with inhibition of inflammatory cytokines and promotion of growth factors.