蛋白质在生命体中发挥着重要的作用, 蛋白质的检测对于了解生命体系及在生物医学领域都有基础性的应用价值。 表面增强拉曼光谱(SERS)技术对研究化学和生物传感应用前景很有优势, 是由于其高灵敏度以及很好的选择性, 并且已被运用于生物分子检测, 特别是在蛋白质检测方面。 综述了SERS探针技术用于蛋白质检验的研究现状, 如免疫胶体金技术、 基于纳米材料探针的SERS研究、 基于酶促技术和基于试剂技术的蛋白质研究进展。

Proteins are essential for living organisms because they are main components of the physiological metabolic pathways of cells. As an ultrasensitive technique, surface-enhanced Raman scatting (SERS) has been proved having a great potential in high throughput biomolecules detection, especially in proteins detection. In this paper, recent advances of the development of SERS probe technology were presented, such as immunogold-based method, Raman-active nanomaterial-based sensors, enzymatic-based sensors, and reagent-based sensors.