光谱学与光谱分析, 2017, 37 (11): 3510, 网络出版: 2018-01-04   

基于全血可见吸收光谱的物种鉴别

Species Identify Based on Visible Absorb Spectrum of Whole Blood
作者单位
中国科学院上海技术物理研究所空间主动光电技术重点实验室, 上海 200083
摘要
血液及血液制品是一种特殊的商品, 在流通过程中需要严加管控。 传统的方法通常为应用特征抗体检测人血红蛋白, 或应用液相色谱(HPLC)、 质谱(MS)等方法对血液物种进行鉴别, 鉴别过程中外界环境和血液样本会发生接触。 为了防止血液样本和外界环境相互污染, 因此需要发展非接触式的血液物种鉴别技术。 设计了一种基于吸收光谱的血液物种鉴别方法。 实验测试了人与牛全血样本共24份, 每种样本各10份, 另有4份标记为未知样本。 装有血液样本的PET管置于积分球内, 使用超连续谱光源对积分球进行照射, 测量420~740 nm范围内匀光后的吸收光谱, 每个样品测试5次取平均。 实验测试了使用主成分分析法对测得的原始光谱数据进行降维。 并对降维后的数据应用二维正态分布进行拟合。 通过样本在置信空间内的概率分布或到已知分类中心的距离对未知样本的物种进行鉴别。 可以得出结论, 应用PCA和二维正态分布拟合的方法, 能够较为精确地实现对全血的可见吸收光谱物种鉴别。
Abstract
Blood and blood products which are special products should be controlled strictly in the process of circulation. The traditional methods to identify the species of the blood use antihuman hemoglobin(Hb) antibodies which recognize and bind to human Hb, or use high-performance liquid chromatography(HPLC) and mass spectrometry (MS). During the identification, the samples may exposure to the external environment. To avoid the pollution of the samples and the environment, a non-connect technique for species of blood identification is needed. The experiment tests 24 human and bovine whole blood samples, including 10 samples for each and 4 unknown samples. The PET tube which contains the blood sample sets in the integrating sphere, which is illuminated by the supercontinuum laser. Each sample takes 5 times of the absorb spectrogram as average in range of 420~740 nm. A PCA method is used to reduce the dimension of original spectral data. Finally, the reduced data are fitted by two-dimensional normal distribution. The species of unknown samples can be identified by using the probability distribution in confidence space or the distance to the known classification center. In conclusion, the visible absorb spectrum of whole blood can be well identified by using the combination of PCA and two-dimensional normal distribution.

刘鹏希, 万雄, 章婷婷. 基于全血可见吸收光谱的物种鉴别[J]. 光谱学与光谱分析, 2017, 37(11): 3510. LIU Peng-xi, WAN Xiong, ZHANG Ting-ting. Species Identify Based on Visible Absorb Spectrum of Whole Blood[J]. Spectroscopy and Spectral Analysis, 2017, 37(11): 3510.

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