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基于L-半胱氨酸组装银纳米棒的SERS的传感器检测汞离子

An Application to Quantitative Analysis of Hg(Ⅱ) with L-Cysteine Molecular Probe by Surface-Enhanced Raman Spectroscopy

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摘要

以L-半胱氨酸(L-Cys)组装银纳米棒的SERS传感器检测汞离子。 讨论了能捕获汞离子的标记分子的种类, 选择L-Cys为标记分子, L-Cys通过S—Ag键链接在银纳米棒表面。 紫外-可见吸收光谱对银纳米棒及组装上L-Cys和Hg2+分别进行表征, 通过10种金属离子验证了该分子探针的对汞离子的特异性吸附, 构建了“Ag-L-Cys-Hg”层状结构。 标记分子-金属纳米粒子偶联物的稳定性由配体分子、 温度、 pH值等决定, 讨论了L-Cys标记分子的浓度、 pH值、 温度的最佳条件, 对一系列汞离子浓度进行测定, 线性范围在0.01~1 μmol·L-1之间, 相关系数为0.990, 检出限为1 nmol·L-1。 对实际水样进行了测定, 加标回收率在85%~103%之间。 建立了一种高效、 快捷、 灵敏度高、 稳定性好痕量测定Hg2+的方法。

Abstract

In this paper, a detection of trace Hg(Ⅱ) was based on silver nanorods by surface-enhanced Raman spectroscopy (SERS)activity. It was discussed the probe types, which explored that the L-cysteine with a high selectivity and sensitivity for Hg(Ⅱ). The UV/Vis spectra was used to characterize the silver nanorods and its modified the L-Cys. Based on L-cysteine of SERS was high sensitivity and selectivity for Hg(Ⅱ) on condition that the ten kinds of metal ions carried on, but only when the single-peak at 1 040 cm-1 structure appeared after adding the Hg(Ⅱ). SERS sensor with L-cysteine assembled silver nanorods firmly captured the Hg(Ⅱ) through the S-Hg bond. It was valuable to get the molecular probe of the concentration, pH and temperature, in which the result showed the optimization when the density of L-cysteine was 1×10-3 mol·L-1 and pH was 7. It did not have a great effect on temperatures, but was down trend over 55 ℃. In order to protect the structure of L-Cysteine and form complexes rapidly, it was selected temperature about 45 ℃. Under the optimized conditions, a series of the concentration of mercury ions were measured, in which the result showed that the density of mercury ions between 0.01 and 5 μmol·L-1 can be analyzed because of a strong peak at 1 040 cm-1 with good linear relationships (correlation=0.990) with the detection limit of 1 nmol·L-1. Which had very excellent sensitivity and stability. When Hg2+ was tested in real water samples, the recovery was from 85%~103%. It establishes a good way to determine the trace Hg(Ⅱ).

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中图分类号:O657.3

DOI:10.3964/j.issn.1000-0593(2018)01-0117-06

基金项目:国家自然科学基金项目(21175015, 21475014)资助

收稿日期:2017-02-28

修改稿日期:2017-07-09

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张彩红:发光与实时分析教育部重点实验室, 西南大学化学化工学院, 重庆 400715
周光明:发光与实时分析教育部重点实验室, 西南大学化学化工学院, 重庆 400715
张璐涛:发光与实时分析教育部重点实验室, 西南大学化学化工学院, 重庆 400715
罗 丹:发光与实时分析教育部重点实验室, 西南大学化学化工学院, 重庆 400715
于 璐:发光与实时分析教育部重点实验室, 西南大学化学化工学院, 重庆 400715
高 意:发光与实时分析教育部重点实验室, 西南大学化学化工学院, 重庆 400715

联系人作者:张彩红(1554943642@qq.com)

备注:张彩红, 女, 1990年生, 西南大学化学化工学院硕士研究生

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引用该论文

ZHANG Cai-hong,ZHOU Guan-ming,ZHANG Lu-tao,LUO Dan,YU Lu,GAO Yi. An Application to Quantitative Analysis of Hg(Ⅱ) with L-Cysteine Molecular Probe by Surface-Enhanced Raman Spectroscopy[J]. Spectroscopy and Spectral Analysis, 2018, 38(1): 117-122

张彩红,周光明,张璐涛,罗 丹,于 璐,高 意. 基于L-半胱氨酸组装银纳米棒的SERS的传感器检测汞离子[J]. 光谱学与光谱分析, 2018, 38(1): 117-122

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