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基于静电富集-表面增强拉曼光谱联用技术的抗生素检测

Detection of Antibiotics Based on Hyphenated Technique of Electrostatic-Preconcentration and Surface-Enhanced-Raman-Spectroscopy

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摘要

利用表面增强拉曼光谱(SERS)和静电富集(EP)相结合的技术,实现了水环境中磺胺甲基嘧啶、丁胺卡那霉素、恩诺沙星和环丙沙星的有效富集和快速痕量探测。实验结果表明,与非静电富集SERS探测相比较,磺胺甲基嘧啶和丁胺卡那霉素的特征峰强度提高了大约10倍,恩诺沙星和环丙沙星的特征峰强度提高了2~3倍;4种抗生素的最低检测浓度分别为1.9×10-8,1.7×10-8,5.5×10-8 ,6.0×10-8 mol·L-1;当被检测目标的浓度较低时,特征峰强度与探测浓度具有良好的线性关系。EP-SERS技术可有效提高水环境中抗生素的检测灵敏度。

Abstract

The effective accumulation and the rapid trace detection of sulfadiazine, amikacin, enrofloxacin and ciprofloxacin are realized by the hyphenated technology of surface enhanced Raman spectroscopy (SERS) and electrostatic preconcentration (EP). The experimental results show that, if compared with those by the non-EP-SERS detection, the characteristic peak intensities of sulfadiazine and amikacin are increased by about 10 times, and the characteristic peak intensities of enrofloxacin and ciprofloxacin are increased by 2-3 times. The minimum detectable concentrations of the 4 kinds of antibiotics are 1.9×10-8, 1.7×10-8, 5.5×10-8 and 6.0×10-8 mol·L-1, respectively. A good linear relationship between the characteristic peak intensity and detection concentration appears when the concentration of detected targets is relatively low. The detection sensitivity of antibiotics in aqueous can be improved effectively by using the EP-SERS technology.

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中图分类号:O433

DOI:10.3788/CJL201845.0207028

所属栏目:“生物医学光子学新技术及进展”专题

基金项目:国家自然科学基金(41476081)、山东省重点研发计划(2016GSF115020)、山东省自然科学基金(BS2013HZ006,ZR2011DQ010

收稿日期:2017-09-13

修改稿日期:2017-10-30

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马海宽:中国海洋大学青岛市光学光电子重点实验室, 山东 青岛 266100
张旭:中国海洋大学青岛市光学光电子重点实验室, 山东 青岛 266100
钟石磊:青岛大学物理科学学院, 山东 青岛 266071
史晓凤:中国海洋大学青岛市光学光电子重点实验室, 山东 青岛 266100
马丽珍:中国海洋大学青岛市光学光电子重点实验室, 山东 青岛 266100
马君:中国海洋大学青岛市光学光电子重点实验室, 山东 青岛 266100

联系人作者:马君(majun@ouc.edu.cn)

备注:马海宽(1989—),男,硕士研究生,主要从事光谱技术及其应用方面的研究。E-mail: dzmahaikuan@163.com

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引用该论文

Ma Haikuan,Zhang Xu,Zhong Shilei,Shi Xiaofeng,Ma Lizhen,Ma Jun. Detection of Antibiotics Based on Hyphenated Technique of Electrostatic-Preconcentration and Surface-Enhanced-Raman-Spectroscopy[J]. Chinese Journal of Lasers, 2018, 45(2): 0207028

马海宽,张旭,钟石磊,史晓凤,马丽珍,马君. 基于静电富集-表面增强拉曼光谱联用技术的抗生素检测[J]. 中国激光, 2018, 45(2): 0207028

被引情况

【1】陈阳,严霞,张旭,史晓凤,马君. 基于支持向量机算法的多环芳烃表面增强拉曼光谱的定量分析. 中国激光, 2019, 46(3): 311005--1

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