中国激光, 2018, 45 (3): 0307009, 网络出版: 2018-03-06   

基于荧光随机涨落的超分辨显微成像 下载: 1482次特邀综述

Fluorescence Fluctuation-Based Super-Resolution Nanoscopy
作者单位
福州大学物理与信息工程学院, 福建 福州 350116
摘要
基于荧光随机涨落的超分辨显微成像技术具有成像速度快、空间分辨率高、系统成本低和光毒性小的成像优势,在对生物亚细胞结构及其动态运动过程的成像和监测中具有广阔的应用前景。近年来,基于荧光发射的间歇性,发展出多种图像重建算法实现荧光涨落超分辨成像,在无须对传统的荧光显微镜做任何硬件改造的情况下,显著提升了光学成像的空间分辨率,实现了突破光学衍射极限的超分辨成像。从重建算法、成像速度、分辨率提升和图像重建质量等方面,对比分析不同类型荧光涨落超分辨方法的差异和适用范围,为生命科学研究人员针对特定的生物学问题选择最佳的超分辨方法提供参考依据。
Abstract
Fluorescence fluctuation-based super-resolution nanoscopy has the imaging advantages of fast imaging, high spatial resolution, low system cost and low phototoxicity, and exhibits broad prospects in imaging and monitoring of biological subcellular structures and dynamics. In recent years, based on fluorescence intermittency of fluorophores, a variety of image reconstruction algorithms have been developed to realize fluorescence fluctuation-based super-resolution imaging. These algorithms can achieve significant resolution enhancement of optical imaging without any hardware modification of conventional fluorescence microscope, and can break the optical diffraction limit. From the perspectives of reconstruction algorithms, imaging speed, resolution improvement and image quality, we analyze and compare the differences and application scopes of multiple types of fluctuation-based nanoscopy, which provides reference on optimal super-resolution techniques selection for researchers in life sciences to investigate specific biological issues.

曾志平. 基于荧光随机涨落的超分辨显微成像[J]. 中国激光, 2018, 45(3): 0307009. Zeng Zhiping. Fluorescence Fluctuation-Based Super-Resolution Nanoscopy[J]. Chinese Journal of Lasers, 2018, 45(3): 0307009.

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