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基于极大倾角光纤光栅的降钙素原免疫传感器

Study on Procalcitonin Immunosensor Based on Excessively Tilted Fiber Grating

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摘要

降钙素原(Procalcitonin, PCT)对于反映患者炎症甚至败血症患病程度具有重要意义。为实现低浓度PCT的免标记检测, 利用极大倾角光纤光栅(Excessively Tilted Fiber Grating, Ex-TFG)的低温度交叉敏感性和高折射率灵敏度的优点, 在光纤的表面修饰金黄色葡萄球菌(Staphylococcus Aureus Protein A, SPA), 再将PCT单克隆抗体固定在SPA分子层, 从而构成对PCT具有特异性检测功能的免疫传感器。实验结果表明: 降钙素原含量为0.5ng/mL时, 谐振波长红移约13pm; PCT的浓度为200ng/mL时, Ex-TFG的红移量基达约125pm; 浓度为1000ng/mL时, 红移量趋于饱和, 最大红移量约150pm。通过对实验数据拟合, 发现Ex-TFG谐振波长的红移量与PCT浓度之间的关系满足Langmuir模型, 相关性系数为0.98。该方法为低浓度PCT的检测提供了一种免标记、快速的检测方法。

Abstract

Procalcitonin (PCT) plays an important role as the factor to reflect patients’ inflammation or sepsis degree. In order to reach a low concentration detection of PCT with label free method, proposed was a type of PCT immunosensor based on excessively tilted fiber grating (Ex-TFG) which is insensitive to temputere but sensitive to refractive index. Staphylococcus aureus Protein A (SPA) was used to modify the surface of Ex-TFG, then highly purified PCT monoclonal antibodies (MAbs) were immobilized on Ex-TFG’s surface through SPA molecules, so as to form the immunosensor which is specific to PCT. Experimental results show that the wavelength shift is about 13pm when PCT concentration is 0.5ng/mL, and it becomes to be 125pm with the concentration of PCT increasing to 200ng/mL. What’s more, the immunosensor becomes saturated at ~1000ng/mL, and the corresponding wavelength shift is about 150pm. Finally, by fitting the experimental data, it demonstrats that the fitting line meets the Langmuir model function with a corelated coefficient of 0.98.

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中图分类号:TN253

DOI:10.16818/j.issn1001-5868.2018.01.003

所属栏目:光电器件

基金项目:国家自然科学基金项目(61505017,51276209);重庆市科委基础与前沿研究项目(cstc2014jcyjA0081,cstc2015jcyjA40035); 中国博士后基金项目(2016M592649);重庆市教委基础/应用基础研究项目(KJ1709192, KJ1709211);重庆理工大学研究生创新基金重点项目(YCX2016103).

收稿日期:2017-08-22

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赵明富:重庆理工大学1. 光纤传感与光电检测重庆市重点实验室
徐杨非:重庆理工大学1. 光纤传感与光电检测重庆市重点实验室
罗彬彬:重庆理工大学1. 光纤传感与光电检测重庆市重点实验室3. 电子科技大学 光电信息学院, 成都 610054
石胜辉:重庆理工大学1. 光纤传感与光电检测重庆市重点实验室
蒋棚俊:药学与生物工程学院, 重庆 400054
鲁 姣:重庆理工大学1. 光纤传感与光电检测重庆市重点实验室
唐 平:重庆理工大学1. 光纤传感与光电检测重庆市重点实验室
吴胜昔:药学与生物工程学院, 重庆 400054

联系人作者:赵明富(zmf@cqut.edu.cn)

备注:赵明富(1964-), 男, 博士, 教授, 硕士生导师, 主要从事信息获取与处理、现代光电检测技术及智能传感器、计算机应用技术等领域的研究工作。

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引用该论文

ZHAO Mingfu,XU Yangfei,LUO Binbin,SHI Shenghui,JIANG Pengjun,LU Jiao,TANG Ping,WU Shengxi. Study on Procalcitonin Immunosensor Based on Excessively Tilted Fiber Grating[J]. Semiconductor Optoelectronics, 2018, 39(1): 10-14

赵明富,徐杨非,罗彬彬,石胜辉,蒋棚俊,鲁 姣,唐 平,吴胜昔. 基于极大倾角光纤光栅的降钙素原免疫传感器[J]. 半导体光电, 2018, 39(1): 10-14

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