激光与光电子学进展, 2018, 55 (3): 033004, 网络出版: 2018-09-10  

具不同碱基数单链核酸的光致发光特性 下载: 1101次

Photoluminescence Properties of Single-Stranded Nucleic Acids with Different Base Sequences
作者单位
1 云南大学物理与天文学院, 云南 昆明 650091
2 中国科学院固体物理研究所, 安徽 合肥 230031
摘要
采用标准光荧光方法,在不加入探针或荧光、磷光染料的情况下,测量了具有56个和29个碱基的两种核酸单链样品的光致发光特性;该方法不会对样品造成破坏或污染。结果表明:当激发波长在310~410 nm之间时,能够在410~480 nm波长区间观测到样品明显的发光峰;两种样品的发光峰随着抽运波长的红移而红移,且发光峰的强度随激发波长的增加而先增强后减弱;具有两种不同碱基数和序列的核酸样品由于具有不同的电子能级结构而显示出了不同的光致发光特性,原理上可以用于甄别两种不同的核酸材料,有助于单链核酸样品的无损、无标记检测和鉴别。
Abstract
We employ the standard fluorescence method to measure the photoluminescence (PL) properties of the single-stranded nucleic acids samples with 56 and 29 base sequences. This method does not need the help of probe and fluorescence or phosphorescence material, does not damage or contaminate the sample during the measurements. It is found that when the excitation wavelength is in the range of 310-410 nm, the PL peak of the samples can be observed in the wavelength range of 410-480 nm. The PL peak red-shifts when the pumping wavelength red-shifts, while the intensity of the PL peak increases and then decreases with the increase of excitation wavelength. The single-stranded nucleic acid samples with two different base sequences show different properties of the PL spectra due to their different electronic band structures. The results are helpful for non-invasively and label-free measurement and identification of nucleic acid materials.

梁昌能, 张杰, 徐文, 丁岚, 钱伟, 李旋. 具不同碱基数单链核酸的光致发光特性[J]. 激光与光电子学进展, 2018, 55(3): 033004. Changneng Liang, Jie Zhang, Wen Xu, Lan Ding, Wei Qian, Xuan Li. Photoluminescence Properties of Single-Stranded Nucleic Acids with Different Base Sequences[J]. Laser & Optoelectronics Progress, 2018, 55(3): 033004.

引用该论文: TXT   |   EndNote

相关论文

加载中...

关于本站 Cookie 的使用提示

中国光学期刊网使用基于 cookie 的技术来更好地为您提供各项服务,点击此处了解我们的隐私策略。 如您需继续使用本网站,请您授权我们使用本地 cookie 来保存部分信息。
全站搜索
您最值得信赖的光电行业旗舰网络服务平台!