为了实现对荧光免疫层析试条浓度的定量测量，设计了一种荧光免疫层析试条成像检测系统。采用365 nm紫外线发光二极管(LED)作为激发光源，通过手持式变倍显微镜相机采集免疫层析试条的荧光图像。根据试条图像荧光检测线和质控线区域位置相对固定的特性，使用最大类间方差遗传算法对图像进行分割，并通过对荧光区域进行定位来去除背景噪声；然后对分割出的荧光图像进行背景滤除，计算出检测线和质控线的灰度值；最后计算荧光区域的特征值，实现对荧光试条浓度的定量检测。实验证明，荧光免疫层析试条成像检测系统的重复性好，5种不同浓度的荧光试条各进行10次重复性检测，检测结果的变异系数均小于0.5%，拟合出的标准曲线的拟合程度可达0.99944，实现了快速定量化检测。

An imaging detection system is designed to achieve quantitative measurement of the concentration of fluorescent immune-chromatographic test strip. Using 365 nm ultraviolet light emitting diode (LED) as the excitation light, we capture the clear image of fluorescent immune-chromatographic test strip by the hand-held zoom microscope camera. Genetic algorithm on the base of Otsu is used to segment the image according to the characteristic of relatively fixed position of the detection line and the quality control line. Then, the background noise is removed by the fluorescence region localization method. The segmented fluorescence image background is filtered out and the gray values of the detection line and the quality control line are calculated. Finally, the eigenvalues of the fluorescence region are calculated to realize the quantitative analysis for the concentration of the fluorescence test strip. The experimental results show that the fluorescence detection system has good repeatability. Five different concentrations of fluorescent test strips are tested for ten times. The variable coefficients of the results are less than 0.5%. The fitting degree of the standard curve reaches 0.99944, and the rapid quantitative detection is realized.