激光生物学报, 2018, 27 (1): 60, 网络出版: 2018-04-23  

水稻-油菜双序列复种免耕、翻耕土壤真菌多样性

Diversity of Filamentous Fungus Community in Paddy Fields with Different Tillage Methods
陈力力 1,2,3,*刘金 1,2,3李梦丹 2,3张立钊 2,3邹应斌 1,2黄敏 1,2
作者单位
1 湖南农业大学, 湖南 长沙 410128
2 南方粮油作物协同创新中心, 湖南 长沙 410128
3 食品科学与生物技术湖南省重点实验室, 湖南 长沙410128
摘要
研究水稻-油菜双序列复种免耕、翻耕稻田土壤真菌的多样性。采用MiSeq 高通量测序方法, 对湖南安乡(AX)、农大(ND)、南县(NX)3个相同处理试验点免耕、翻耕土壤样品进行ITS 区高通量测序、多样性比较分析、物种组成分析及样品聚类分析。结果表明6个样品共得到真菌优化序列221 105条, 分属于1 356个OTU, 真菌种类覆盖7门、121科、230属; 指数分析表明NT样品菌群相对丰度及群落多样性均略高于CT样品; 在门水平上各试验点菌群结构相似, 前三位为子囊菌门、接合菌门和担子菌门, 但是同一试验点NT样品子囊菌相对丰度均低于CT样品, 接合菌和担子菌相对丰度均高于CT样品。科属水平比较表明, 3个试验点NT样品被孢霉科相对丰度比CT样品的大3-5倍, 毛壳菌科的相对丰度小1-2倍; NT样品Carpoligna、栓菌属(Trametes)、网孢菌属 (Filobasidium)、被孢霉属(Mortierella)的序列数明显大于CT样品, 毛壳属(Chaetomium)、柄孢壳菌属(Podospora)及瓜亡革菌属(Thanatephorus)的序列数明显小于CT样品。土壤理化因子测定表明NT样品的有机质、全氮及速效钾含量均高于CT样品。本研究得出水稻-油菜双序列复种免耕、翻耕稻田土壤样品真菌物种组成丰富, 不同耕作方式土壤真菌种群结构存在差异, 菌群组成及丰度对土壤理化因子有一定影响。
Abstract
To study the diversity and community structure of filamentous fungus in rice-oilseed multiple cropping fields with convertional-tillage(CT) and no-tillages(NT). Micro-organism population analysis were studied based on ITS sequences analyzed by MiSeq. The results showed that 221 105 vaild sequences was obtained, it covered 1 356 OTU, 7 phylums, 121 families and 230 generaes; The index analysis showed that the relative abundance and community diversity of the NT samples were slightly higher than that of the CT samples. On the phylums level, the relative abundance of Ascomycota in NT samples was lower than that in CT and the zygotes and basidites was higher than that in CT; The vaild sequences of Carpoligna、Trametes、Filobasidium、Mortierella in NT samples was higher than that in CT and Chaetomium、Podospora、Thanatephorus was lower than that in CT on the generaes level. The physical and chemical factors of soil organic matter determination showed that the total nitrogen and available potassium content in NT samples were higher than those in CT samples. There are indifferences filamentous fungus population structure in rice-oilseed multiple cropping fields with convertional-tillage(CT) and rice-oilseed multiple cropping and no-tillages(NT).The composition and abundance of flora has a certain effect on the soil physical and chemical factors.

陈力力, 刘金, 李梦丹, 张立钊, 邹应斌, 黄敏. 水稻-油菜双序列复种免耕、翻耕土壤真菌多样性[J]. 激光生物学报, 2018, 27(1): 60. CHEN Lili, LIU Jin, LI Mengdan, ZHANG Lizhao, ZOU Yingbin, HUANG Min. Diversity of Filamentous Fungus Community in Paddy Fields with Different Tillage Methods[J]. Acta Laser Biology Sinica, 2018, 27(1): 60.

关于本站 Cookie 的使用提示

中国光学期刊网使用基于 cookie 的技术来更好地为您提供各项服务,点击此处了解我们的隐私策略。 如您需继续使用本网站,请您授权我们使用本地 cookie 来保存部分信息。
全站搜索
您最值得信赖的光电行业旗舰网络服务平台!