MicroRNA(miRNA)与疾病的发生和发展密切相关, 可作为疾病诊断标志物。常规检测miRNA的方法耗时较长且操作复杂, 本文设计了一种便携式miRNA快速检测系统。该系统采用光电检测技术检测滚环扩增后的标志物miRNA受激发出的荧光强度, 对采集到的荧光数据进行分析, 得出miRNA的变化情况。实验得出便携式miRNA快速检测系统在试剂浓度为0.1~1 μmol时, 荧光强度线性偏倚不超过±5%, 系统重复性大于95%。通过对冠心病检测标志物miR-499进行滚环扩增实验, 结果表明设计的便携式miRNA快速检测系统能够快速有效地检测miRNA。

MicroRNA (miRNA) is closely related to the occurrence and development of certain diseases and can be used as a diagnostic marker. Conventional methods for the detection of miRNA are time-consuming and complex. Herein, a portable rapid miRNA detection system was designed. This system records the fluorescence intensity of the marker that is amplified via rolling circle amplification using a photoelectric detection technique, and analyzes the fluorescence data to obtain the changes in miRNA. Through our experiments, we observed that for reagent concentrations in the range 0.1-1 μmol, the linearity of the fluorescence intensity detected by the miRNA fluorescence detection equipment was less than ±5%, and the repeatability was ≥95%. Our roll amplification experiment involving the coronary heart disease diagnostic marker, miR-499, confirmed that the portable rapid miRNA detection system can detect miRNA quickly and effectively.