目前应用激光热疗治疗肿瘤的研究比较多, 但是激光联合光敏剂治疗肿瘤过程中肿瘤组织的三维温度分布的研究比较少。为了分析激光热疗过程中肿瘤及周围组织的三维温度分布情况, 本实验首先运用红外热像仪监测激光联合吲哚菁绿治疗荷乳腺瘤小鼠过程中肿瘤表面的温度变化; 其次, 运用蒙特卡洛(Monte Carlo)原理模拟激光在生物组织中的光分布, 进一步利用COMSOL Multiphysics构建激光热疗过程中肿瘤及周围组织温度的三维分布仿真模型。结果显示实际测量肿瘤表面最高点温度和仿真模拟肿瘤表面最高点温度分布吻合度较好; 有光敏剂的肿瘤组织温度比没有光敏剂的肿瘤组织温度升高约20 ℃; 模型中可见肿瘤组织不同部位温度可相差15 ℃左右。该模型可以分析肿瘤以及周围组织的温度时空分布情况, 了解激光热疗治疗肿瘤的效果, 该模型为激光热疗的应用提供参考作用。

There are many studies about photothermal therapy for treatment of tumor. However, there are few studies about three-dimensional temperature distribution of tumor tissue during the treatment of laser combined with photosensitizer. This study aimed at constructing three-dimensional temperature distribution of tumor tissue in tumor bearing mice during the process of photothermal therapy. Firstly, the surface temperature of tumor tissue was monitored by near infrared thermometer during the treatment of laser combined with indocyanine green. Secondly, based on the Monte Carlo principle which could simulate laser light distribution in biological tissues, COMSOL Multiphysics analysis software was used to construct temperature distribution simulation model of the tumor tissue during the photothermal therapy. The results revealed that the temperature measured during the photothermal therapy and the temperature calculated by simulation model are consistent. The temperature of tumor tissue with photosensitizer was higher about 20 ℃ than that without photosensitizer. The temperature elevation was about 15 ℃ between the high temperature part of tumor tissue and the low temperature part of tumor tissue. This simulation is capable to analyze the spatial and temporal distribution of temperature in tumor tissues and surrounding tissues and holds great potential in the application of photothermal therapy.