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扫频光学相干层析成像应用于判断黏液型胃癌边界区域

Boundary Region of Stomach Mucinous Carcinoma with Swept Source Optical Coherence Tomography

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摘要

为了在癌症外科手术中提供实时反馈,更精确地切除癌症组织,从而在术中导航领域展现应用潜力,利用光学相干层析成像(OCT)系统对39例胃癌患者肿瘤组织成像,并与对应位置的病理切片进行对比。结果表明:有效的38个病例的OCT图像能清晰地分辨癌症组织与正常组织的差别,其中有4例黏液型胃癌,黏液型胃癌的肿瘤分泌的黏液会破坏胃组织的层状结构,导致纹理紊乱,黏膜残缺;黏液对光的背向散射更弱以及与黏膜组织的背向散射对比相差更大使得黏液型胃癌的OCT图像成像更清晰,B型超声扫描(B-scan)的OCT图像能够分辨该种类型癌症的边界区域;将B-scan的OCT图像进行三维(3D)重建后显示整个成像区域的体结构,经过表面展平后提取深度方向上不同层的切面图像,提供了另一维度视角观察胃癌组织的边界区域,与B-scan图像交叉验证可以显示优异的区分效果。

Abstract

To provide a real-time intraoperative feedback and suggest an applied potential to guide stomach cancer surgery for removal of normal tissue as little as possible, we use an optical coherence tomography (OCT) to image normal tissue and tumor tissue of 39 stomach cancerous patients, and compare OCT images and pathological sections. The results show that OCT images of 38 patients show obvious difference between normal tissue and tumor tissue. For the 4 stomach mucinous carcinomas, the mucus secreted by tumor of stomach mucinous carcinoma destroys the layer structure, texture, and mucosa of stomach tissue. The poorer backscattering of mucus and the greater difference between backscattering of mucosal tissue make the OCT images of stomach mucinous carcinoma clearer. The B-mode ultrasonic scanning (B-scan) OCT images can distinguish the boundary of the cancerous tissue and the normal tissue. A volumetric image can be built with B-scan images by the three-dimensional (3D) reconstruction method. The layered section images in tissue-depth direction are obtained after volumetric surface flattening. These images provide another dimensionality to view stomach cancerous boundary. The cross validation with B-scan images can show excellent distinction.

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中图分类号:TN247

DOI:10.3788/aos201838.0517001

所属栏目:医用光学与生物技术

基金项目:国家自然科学基金(61575107)、清华大学自主创新项目

收稿日期:2017-11-16

修改稿日期:2017-12-24

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作者单位    点击查看

罗斯特:清华大学电子工程系, 北京 100084
范应威:清华大学生物医学工程系, 北京 100084
常玮:清华大学生物医学工程系, 北京 100084
安昕:中国科学院苏州纳米技术与纳米仿生研究所, 江苏 苏州 215123佛山市欧谱曼迪科技有限公司, 广东 佛山 528000
赵辉:佛山市欧谱曼迪科技有限公司, 广东 佛山 528000
霍力:清华大学电子工程系, 北京 100084

联系人作者:霍力(lhuo@mail.tsinghua.edu.cn)

备注:罗斯特(1987-),男,博士研究生,主要从事生物光子学、医学成像方面的研究。E-mail: lost147258@163.com

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引用该论文

Luo Site,Fan Yingwei,Chang Wei,An Xin,Zhao Hui,Huo Li. Boundary Region of Stomach Mucinous Carcinoma with Swept Source Optical Coherence Tomography[J]. Acta Optica Sinica, 2018, 38(5): 0517001

罗斯特,范应威,常玮,安昕,赵辉,霍力. 扫频光学相干层析成像应用于判断黏液型胃癌边界区域[J]. 光学学报, 2018, 38(5): 0517001

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