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贝塞尔光束的互补光束设计与优化

Construction and Optimization of the Complementary Beam of Bessel Beam

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摘要

提出无衍射贝塞尔光束的互补光束概念.基于惠更斯-菲涅尔原理和光场的相干叠加性, 给出了其频谱的一般形式.利用遗传算法搜索频谱相位分布的最优解, 使得扫描互补光束光片场的强度分布与贝塞尔光片旁瓣强度分布几乎一致, 二者相减可消除旁瓣的影响, 从而获得大视场均匀的理想薄光片.将其应用在光片荧光显微成像中, 可有效去除离焦背景噪声, 提高成像质量.

Abstract

The idea of propagation-invariant Complementary Beam (CB) to Bessel beam was proposed. Based on the Huygens-Fresnel principle and the superposition of coherent light field, the general form of CB′s spectrum is suggested. The phase distribution of complex amplitude spectrum is optimized with the aid of genetic algorithm, in doing so the scanning complementary beam light-sheet enables to approximate the side lobes of Bessel light-sheet to the utmost. Thus, the substraction of two scans can eliminate the influence of the side lobes to produce a large FOV, homogeneous and thin light-sheet. The employment of the complementary beam into light-sheet fluorescence microscopy is a promising method to reduce the background signal as well as enhance the imaging quality.

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中图分类号:O439

DOI:10.3788/gzxb20184707.0708002

基金项目:国家自然科学基金(Nos. 81427802, 11704405), 中国科学院“西部青年学者”项目(No. XAB2016B26)和西安市博士后创新基地科研项目资助

收稿日期:2018-03-27

修改稿日期:2018-05-03

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作者单位    点击查看

贾昊:中国科学院西安光学精密机械研究所 瞬态光学与光子技术国家重点实验室, 西安 710119中国科学院大学, 北京 100049
于湘华:中国科学院西安光学精密机械研究所 瞬态光学与光子技术国家重点实验室, 西安 710119
杨延龙:中国科学院西安光学精密机械研究所 瞬态光学与光子技术国家重点实验室, 西安 710119
周兴:中国科学院西安光学精密机械研究所 瞬态光学与光子技术国家重点实验室, 西安 710119中国科学院大学, 北京 100049
严绍辉:中国科学院西安光学精密机械研究所 瞬态光学与光子技术国家重点实验室, 西安 710119
刘超:中国科学院西安光学精密机械研究所 瞬态光学与光子技术国家重点实验室, 西安 710119中国科学院大学, 北京 100049
雷铭:中国科学院西安光学精密机械研究所 瞬态光学与光子技术国家重点实验室, 西安 710119
姚保利:中国科学院西安光学精密机械研究所 瞬态光学与光子技术国家重点实验室, 西安 710119

联系人作者:贾昊(jiahao@opt.cn)

备注:贾昊(1990-), 男, 硕士研究生, 主要研究方向为光片荧光显微技术.

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引用该论文

JIA Hao,YU Xiang-hua,YANG Yan-long,ZHOU Xing,YAN Shao-hui,LIU Chao,LEI Ming,YAO Bao-li. Construction and Optimization of the Complementary Beam of Bessel Beam[J]. ACTA PHOTONICA SINICA, 2018, 47(7): 0708002

贾昊,于湘华,杨延龙,周兴,严绍辉,刘超,雷铭,姚保利. 贝塞尔光束的互补光束设计与优化[J]. 光子学报, 2018, 47(7): 0708002

被引情况

【1】于湘华,刘超,柏晨,杨延龙,彭彤,但旦,闵俊伟,姚保利. 光片荧光显微成像技术及应用进展. 激光与光电子学进展, 2020, 57(10): 100001--1

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