Journal of Innovative Optical Health Sciences, 2018, 11 (1): 1850007, Published Online: Sep. 17, 2018  

Rapid bacteria identification using structured illumination microscopy and machine learning

Author Affiliations
1 College of Engineering, Huazhong Agricultural University, Wuhan 430070, P. R. China
2 College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, P. R. China
3 State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, P. R. China
4 Bio-Medical Center, Huazhong Agricultural University, Wuhan 430070, P. R. China
5 Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, P. R. China
Abstract
Traditionally, optical microscopy is used to visualize the morphological features of pathogenic bacteria, of which the features are further used for the detection and identification of the bacteria. However, due to the resolution limitation of conventional optical microscopy as well as the lack of standard pattern library for bacteria identification, the effectiveness of this optical microscopybased method is limited. Here, we reported a pilot study on a combined use of Structured Illumination Microscopy (SIM) with machine learning for rapid bacteria identification. After applying machine learning to the SIM image datasets from three model bacteria (including Escherichia coli, Mycobacterium smegmatis, and Pseudomonas aeruginosa), we obtained a classification accuracy of up to 98%. This study points out a promising possibility for rapid bacterial identification by morphological features.

Yingchuan He, Weize Xu, Yao Zhi, Rohit Tyagi, Zhe Hu, Gang Cao. Rapid bacteria identification using structured illumination microscopy and machine learning[J]. Journal of Innovative Optical Health Sciences, 2018, 11(1): 1850007.

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