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基于时域荧光寿命的水体溶解氧浓度检测方法

Detection Method of Dissolved Oxygen Concentration in Water Based on Time-Domain Fluorescence Lifetime

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摘要

依据氧气对物质荧光的猝灭作用,研究了一种基于时域荧光寿命的水体溶解氧浓度检测方法,根据荧光猝灭曲线上的两点计算荧光寿命,并通过Stern-Volmer方程反演获得水体溶解氧浓度。结果表明:相同溶解氧浓度下,归一化处理后的荧光猝灭曲线不受激发光强度和激励持续时间等激发条件的影响;不同溶解氧浓度下,实测荧光寿命受系统延迟的影响,补偿后荧光寿命理论曲线与修正曲线具有良好的一致性,拟合相关系数达0.9985。与HQ30d溶解氧分析仪对比,测试结果表明,0~20 mg·L-1范围内溶解氧质量浓度测量误差小于0.5 mg·L-1,线性相关系数达0.9992。

Abstract

On the basis of the quenching effect of oxygen on the material fluorescence, we developed an analytical method based on time-domain fluorescence lifetime to detect the concentration of dissolved oxygen in water. The fluorescence lifetime is calculated according to two points on the fluorescence quenching curve, and the dissolved oxygen concentration in water is then obtained by the inversion of Stern-Volmer equation. The results showed that under the same dissolved oxygen concentration, the normalized fluorescence quenching curve is not affected by the excitation conditions, such as the intensity of the excitation light and the duration of excitation. The measured fluorescence lifetime of different dissolved oxygen concentrations is affected by the system delay. The theoretical curve of the fluorescence lifetime after compensation is in good agreement with the modified curve with the fitting correlation coefficient of 0.9985. Compared with the HQ30d dissolved oxygen analyzer, the measurement error of the dissolved oxygen in the mass concentration range of 0-20 mg·L-1 is less than 0.5 mg·L-1 while the fitting correlation coefficient reach to 0.9992. These results showed that it could be an effective method for the measurement of dissolved oxygen concentration in water.

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中图分类号:X832

DOI:10.3788/aos201838.1001005

所属栏目:大气光学与海洋光学

基金项目:国家重点研发计划项目(2016YFC1400604)、青岛海洋科学与技术国家实验室开放基金项目(QNLM2016ORP0312)、安徽省重点研发计划项目(1804a0802192)

收稿日期:2018-03-27

修改稿日期:2018-04-27

网络出版日期:2018-05-21

作者单位    点击查看

涂梦迪:中国科学院安徽光学精密机械研究所环境光学与技术重点实验室, 安徽 合肥 230031中国科学技术大学, 安徽 合肥 230026
殷高方:中国科学院安徽光学精密机械研究所环境光学与技术重点实验室, 安徽 合肥 230031
赵南京:中国科学院安徽光学精密机械研究所环境光学与技术重点实验室, 安徽 合肥 230031
覃志松:桂林电子科技大学计算机与信息安全学院, 广西 桂林 541004
王翔:中国科学院安徽光学精密机械研究所环境光学与技术重点实验室, 安徽 合肥 230031中国科学技术大学, 安徽 合肥 230026
董鸣:中国科学院安徽光学精密机械研究所环境光学与技术重点实验室, 安徽 合肥 230031
刘建国:中国科学院安徽光学精密机械研究所环境光学与技术重点实验室, 安徽 合肥 230031
刘文清:中国科学院安徽光学精密机械研究所环境光学与技术重点实验室, 安徽 合肥 230031

联系人作者:赵南京(njzhao@aiofm.ac.cn); 涂梦迪(mdtu@aiofm.ac.cn);

【1】Wang J, Zhang F, Wang X P, et al. Three-dimensional fluorescence characteristics by parallel factor method coupled with self-organizing map and its relationship with water quality[J]. Acta Optica Sinica, 2017, 37(7): 0730003.
王娟, 张飞, 王小平, 等. 平行因子法结合自组织映射神经网络的三维荧光特征及其与水质的关系[J]. 光学学报, 2017, 37(7): 0730003.

【2】Hitchman M L. Measurement of dissolved oxygen[M]. Geneva: Orbisphere Lab., 1978: 255.

【3】Standard Test Methods for Dissolved Oxygen in Water: Designation: D 888-92[S]. Pennsylvania: ASTM, 1996.

【4】Wang X D, Wolfbeis O S. Optical methods for sensing and imaging oxygen: materials, spectroscopies and applications[J]. Chemical Society Reviews, 2014, 43(10): 3666-3761.

【5】Wolfbeis O S. Luminescent sensing and imaging of oxygen: fierce competition to the Clark electrode[J]. Bioessays, 2015, 37(8): 921-928.

【6】Pensieri S, Bozzano R, Schiano M E, et al. Methods and best practice to intercompare dissolved oxygen sensors and fluorometers/turbidimeters for oceanographic applications[J]. Sensors, 2016, 16(5): 702.

【7】Feng W W, Zhou N, Chen L X, et al. An optical sensor for monitoring of dissolved oxygen based on phase detection[J]. Journal of Optics, 2013, 15(5): 055502.

【8】Liu C, Zhou Y, Wang X W, et al. Fluorescence lifetime imaging microscopy and its research progress[J]. Laser & Optoelectronics Progress, 2011, 48(11): 111102.
刘超, 周燕, 王新伟, 等. 荧光寿命成像技术及其研究进展[J]. 激光与光电子学进展, 2011, 48(11): 111102.

【9】McDonagh C, Kolle C, McEvoy A K, et al. Phase fluorometric dissolved oxygen sensor[J]. Sensors and Actuators B: Chemical, 2001, 74(1/2/3): 124-130.

【10】Zhang T. Design of a in-situ dissolved oxygen detector using fluorescence quenching[D]. Hangzhou: Zhejiang University, 2013.
张涛. 海洋原位溶解氧检测仪设计与开发[D]. 杭州: 浙江大学, 2013.

【11】Danielson S J D, Baltz N T. Phase shift measurement for luminescent light: US20070029505[P]. 2007-08-01.

【12】Xu J G, Wang Z B. Fluorescence analysis[M]. 3rd ed. Beijing: Science Press, 2006: 64-86.
许金钩, 王尊本. 荧光分析法[M]. 3版. 北京: 科学出版社, 2006: 64-86.

【13】Li D X, Xu X, Li N, et al. Time-resolved fluorescence technique and fluorescence lifetime measurement[J]. University Chemistry, 2008, 23(4): 1-11.
李东旭, 许潇, 李娜, 等. 时间分辨荧光技术与荧光寿命测量[J]. 大学化学, 2008, 23(4): 1-11.

引用该论文

Tu Mengdi,Yin Gaofang,Zhao Nanjing,Qin Zhisong,Wang Xiang,Dong Ming,Liu Jianguo,Liu Wenqing. Detection Method of Dissolved Oxygen Concentration in Water Based on Time-Domain Fluorescence Lifetime[J]. Acta Optica Sinica, 2018, 38(10): 1001005

涂梦迪,殷高方,赵南京,覃志松,王翔,董鸣,刘建国,刘文清. 基于时域荧光寿命的水体溶解氧浓度检测方法[J]. 光学学报, 2018, 38(10): 1001005

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