We have used a gold nanohole array to trap single polystyrene nanoparticles, with a mean diameter of 30 nm, into separated hot spots located at connecting nanoslot regions. A high trap stiffness of approximately 0.85 fN/(nm·mW) at a low-incident laser intensity of ～0.51 mW/μm2 at 980 nm was obtained. The experimental results were compared to the simulated trapping force, and a reasonable match was achieved. This plasmonic array is useful for lab-on-a-chip applications and has particular appeal for trapping multiple nanoparticles with predefined separations or arranged in patterns in order to study interactions between them.