快速、准确评估浮游植物的初级生产力对研究海洋生态环境科学及理解全球碳循环演变规律等至关重要。针对 ¹⁴C示踪法和黑白瓶法等传统方法表示的初级生产力存在测量周期长、操作繁琐等问题,依据生物膜能流理论,基于荧光动力学方法,即通过光源诱导产生可变叶绿素荧光获得光合参数,结合光合电子传递速率“生物-光学”模型,对基于荧光动力学参数的光合电子传递速率的测量进行研究。通过不同胁迫条件下蛋白核小球藻的测试,对比液相氧电极测量的光合放氧速率,验证光合电子传递速率测量浮游植物初级生产力的有效性。结果表明:在不同浓度的二氯苯基二甲脲的胁迫下,浮游植物的光合电子传递速率与光合放氧速率具有良好的一致性,且二者随胁迫浓度增加而减小明显,光合放氧速率与光合电子传递速率分别减小了71.55%和68.87%,二者相关系数的平方达到0.934;在不同营养盐或光照强度下胁迫培养15 d,浮游植物的光合电子传递速率与光合放氧速率仍然具有良好的一致性,二者相关系数的平方大于0.955。

Rapid and accurate assessment of the primary productivity of phytoplankton is crucial to scientific research of marine ecological environment and understanding of evolution rules of global carbon cycle. The measurement period of primary productive forces represented by the 14C tracer method or the black and white bottle method is long and cumbersome. To solve the problem, we study the photosynthetic electron transfer rate according to the biofilm energy flow theory. Photosynthetic parameters are obtained by the variable chlorophyll fluorescence induced by light source, and combined with photosynthetic electron transfer rate “biological-optics”. Therefore, the measurement of photosynthetic electron transfer rate based on fluorescence kinetic parameters is studied. The chlorella pyrenoidosa is tested under different stress conditions, and the photosynthetic oxygen rate measured by the liquid phase oxygen electrode is compared to verify effectiveness of the photosynthetic electron transfer rate. The results show that under different concentrations of dichlorophenyl dimethylurea stress, the photosynthetic electron transport rate and photosynthetic oxygen release rate of phytoplankton have good consistency, and both decrease significantly with the increase of the stress concentration. The photosynthetic oxygen release rate and photosynthetic electron transport rate are reduced by 71.55% and 68.87%, respectively, and their square of correlation coefficient is 0.934. Under different nutrients or light intensity for 15 d, the photosynthetic electron transport rate and photosynthetic oxygen release rate still have good consistency, and their square of correlation coefficient is greater than 0.955.