首页 > 论文 > 半导体光电 > 39卷 > 5期(pp:634-639)

基于星形金纳米颗粒修饰的81°倾斜光纤光栅生物传感器

Biosensor Based on 81° Tilted Fiber Grating Modified By StarShaped Au Nanoparticles

  • 摘要
  • 论文信息
  • 参考文献
  • 被引情况
  • PDF全文
分享:

摘要

提出一种星形金纳米颗粒修饰的81°倾斜光纤光栅(TFG)生物传感器。通过金黄色葡萄球菌蛋白A固定法, 将自制的高纯度新城疫病毒(NDV)单克隆抗体固定于81°TFG表面, 制成对NDV具有特异性检测功能的生物传感器。结果表明, 传感器对NDV的最低探测极限达10~25pg/mL, 检测饱和点约为1000pg/mL; 在0~200pg/mL具有较好的线性相关度(R2约为0.911), 相应的灵敏度约为1394pm/(ng/mL)。此外, 通过对NDV的特异性和临床性测试, 表明该生物传感器对NDV具有高度的特异性和临床有效性。

Abstract

A type of biosensor based on 81° tilted fiber grating (81° TFG) modified by star shaped Au nanoparticles (StAuNP) was proposed. Highly purified newcastle disease virus (NDV) monoclonal antibodies (MAb) were immobilized on sensor surface by using staphylococcus aureus protein A (SPA) method, so as to form the biosensor specific to NDV. Experimental results show that, the proposed biosensor has a lowest detection limit (LOD) of 10~25pg/mL for NDV and become saturated at ~1000pg/mL, and it has a linearity sensitivity of ~1394pm/(pg/mL) with R2 of ~0.911 in the low concentration range of 0~200pg/mL. In addition, through the specificity and clinical test, the proposed biosensor shows very high specificity to NDV and clinical effectiveness.

广告组1 - 空间光调制器+DMD
补充资料

中图分类号:TN253

DOI:10.16818/j.issn1001-5868.2018.05.006

所属栏目:光电器件

基金项目:国家自然科学基金项目(61505017); 中国博士后基金项目(2016M592649); 重庆市教委科学技术研究项目(KJ1709192, KJ1709211)

收稿日期:2018-03-21

修改稿日期:--

网络出版日期:--

作者单位    点击查看

罗彬彬:电子科技大学 光电科学与工程学院, 成都 610054重庆理工大学 光纤传感与光电检测重庆市重点实验室, 重庆 400054
赵明富:重庆理工大学 光纤传感与光电检测重庆市重点实验室, 重庆 400054
石胜辉:重庆理工大学 光纤传感与光电检测重庆市重点实验室, 重庆 400054
刘 永:电子科技大学 光电科学与工程学院, 成都 610054

联系人作者:罗彬彬(luobinbin@cqut.edu.cn)

备注:罗彬彬(1981-), 男, 博士, 硕士生导师, 副教授, 主要从事光纤光栅传感器方面的研究。

【1】Zhou K, Zhang L, Bennion I, et al. Optic sensors of high refractiveindex responsivity and low thermal cross sensitivity that use fiber Bragg gratings of >80° tilted structures[J]. Opt. Lett., 2006, 31(9): 11931195.

【2】Zhou K, Chen X, Zhang L, et al. Low thermal sensitivity grating devices based on Ex45° tilting structure capable of forwardpropagating cladding modes coupling[J]. J. Lightwave Technol., 2006, 24(12): 50875094.

【3】Yan Z, Sun Z, Zhou K, et al. Numerical and experimental analysis of sensitivityenhanced RI sensor based on 81° TFG in thin cladding fiber[J]. J. Lightwave Technol., 2015, 33(14): 30233027.

【4】Chen X, Zhou K, Zhang L, et al. Infiber twist sensor based on a fiber Bragg grating with 81° tilted structure[J]. Photon. Technol. Lett., 2006, 18(24): 25962598.

【5】Mou C, Zhou K, Yan Z, et al. Liquid level sensor based on an excessively tilted fibre grating[J]. Opt. Commun., 2013, 30(1): 271275.

【6】罗彬彬, 邹文根, 赵明富, 等. 极大倾斜角度光纤光栅pH值传感器及其增敏研究[J]. 光学学报, 2017, 37(1): 010600918.
Luo B B, Zou W G, Zhao M F, et al. pH sensor based on fiber grating with extremely large tilt angle and its sensitivity enhancement[J]. Act. Opt. Sin., 2017, 37(1): 010600918.

【7】Luo B, Yan Z, Sun Z, et al. Novel glucose sensor based on enzyme immobilized 81° tilted fiber grating[J]. Opt. Express, 2014, 22(25): 3057130578.

【8】Luo B, Yan Z, Sun Z, et al. Biosensor based on excessively tilted fiber grating in thincladding optical fiber for sensitive and selective detection of low glucose concentration[J]. Opt. Express, 2015, 23(25): 3242932440.

【9】Luo B, Wu S, Zou W, et al. Labelfree immunoassay for porcine circovirus type 2 based on excessively tilted fiber grating modified with staphylococcal protein A[J]. Biosensors and Bioelectron., 2016, 86: 10541060.

【10】Luo B, Wu S, Zhang Z, et al. Human heart failure biomarker immunosensor based on excessively tilted fiber gratings[J]. Biomedical Opt. Express, 2017, 8(1): 5767.

【11】罗彬彬, 吴胜昔, 王玲玲, 等. 基于81°倾斜光纤光栅的新城疫病毒免疫传感器[J]. 光学学报, 2017, 37(11): 1106001.
Luo B B, Wu S X, Wang L L, et al. Study on newcastle disease virus immunosensor based on 81° tilted fiber grating[J]. Act. Opt. Sin., 2017, 37(11): 1106001.

【12】Yan Z, Wang H, Wang C, et al. Theoretical and experimental analysis of excessively tilted fiber gratings[J]. Opt. Express, 2016, 24(11): 1210712115.

【13】Zhang Q, Xue C, Yuan Y, et al. Fiber surface modification technology for fiberoptic localized surface plasmon resonance biosensors[J]. Sensors, 2012(12): 27292741.

【14】Sjoquist J, Meloun B, Hjelm H, et al. Protein A isolate from staphylococcus aureus after digestion with lysostaphin[J]. Eur. J. Biochem., 1972, 29(3): 572578.

【15】Kangwa M, Yelemane V, Polat A N, et al. Highlevel fedbatch fermentative expression of an engineered staphylococcal protein A based ligand in E. coli: purification and characterization[J]. AMB Express, 2015, 5(1): 7079.

引用该论文

LUO Binbin,ZHAO Mingfu,SHI Shenghui,LIU Yong. Biosensor Based on 81° Tilted Fiber Grating Modified By StarShaped Au Nanoparticles[J]. Semiconductor Optoelectronics, 2018, 39(5): 634-639

罗彬彬,赵明富,石胜辉,刘 永. 基于星形金纳米颗粒修饰的81°倾斜光纤光栅生物传感器[J]. 半导体光电, 2018, 39(5): 634-639

您的浏览器不支持PDF插件,请使用最新的(Chrome/Fire Fox等)浏览器.或者您还可以点击此处下载该论文PDF