光谱学与光谱分析, 2018, 38 (10): 3023, 网络出版: 2018-11-25  

基于OPO脉冲激发的血糖光声检测影响因素研究

Studies on the Influence Factors of the Blood Glucose Photoacoustic Measurement Based on OPO Pulsed Laser Induction
作者单位
江西科技师范大学光电子与通信重点实验室, 江西 南昌 330038
摘要
构建了一套基于侧向模式的光学参数振荡(optical parameter oscillator, OPO)脉冲激光激发-超声传感的血糖光声检测系统, 并且将激发波长、 激光能量、 探测频率和温度等对血糖光声检测的影响, 在系统结构上进行了有效融合。 然后, 以不同浓度的葡萄糖溶液作为测试样品, 实验研究了几个因素(激发波长、 激光能量、 探测频率和温度等)对葡萄糖光声检测和浓度预测的影响。 实验得到了不同浓度葡萄糖溶液在不同影响因素条件下的葡萄糖实时光声信号和光声峰峰值, 同时, 为了得到不同因素对葡萄糖光声值和浓度预测的影响规律, 通过线性回归算法建立了葡萄糖光声峰峰值与各因素变化, 以及不同因素下光声峰峰值与浓度梯度的映射关系模型。 实验和模型预测结果表明: 相比1 200和1 300 nm波长而言, 1 064 nm波长下的葡萄糖光声峰峰值与浓度梯度模型预测效果最好, 模型相关系数达到0.986; 葡萄糖光声峰峰值随着脉冲激光输出能量的增大呈线性增大趋势, 且葡萄糖浓度预测准确度随着激光输出能量增大有所提高; 根据不同探测频率的超声探测器捕获的葡萄糖光声峰峰值与浓度梯度建模效果来看, 响应频率为1 MHz的超声探测器相比2.5和10 MHz的超声探测器, 在葡萄糖光声信号探测上具有更好的探测效果; 最后, 实验发现, 葡萄糖光声峰峰值随着温度和浓度的增大均呈线性增大趋势, 且浓度预测误差随着温度的升高逐渐增大。
Abstract
In this paper, in order to ascertain the effects and influence laws of some factors on the photoacoustic detection of blood glucose, we firstly established a set of a blood glucose photoacoustic measurement system based on the optical parameters oscillators (OPO) pulsed laser induced ultrasonic detection in the lateral mode. In this system, the wavelength tunable pulsed laser was used as the excitation source of photoacoustic signals of blood glucose, and the ultrasonic transducer with high sensitivity was used to capture the photoacoustic signals of blood glucose. Moreover, the lateral detection mode was built because the irradiation direction of pulsed laser was perpendicular to the detection direction of ultrasonic transducer, which greatly overcame the interference of irradiation light directly penetrated the test solutions into the surface of ultrasonic transducer for the photoacoustic signals of samples. In addition, in the system, the effects of several factors on the photoacoustic detection of blood glucose were combined from the viewpoint of structural design. Then, the glucose solutions with different concentrations were used as the test samples. The effects of several factors (excitation wavelength, laser output energy, detection frequency and temperature) on the photoacoustic detection of glucose and concentration prediction were experimentally investigated. Besides, the time-resolved photoacoustic signals and photoacoustic peak-to-peak values of glucose with different concentrations under the different influence factors were experimentally obtained. At the same time, in order to know the influence laws of factors on the photoacoustic values and concentration prediction of glucose, the linear fitting algorithm was used to establish the models between the photoacoustic peak-to-peak values and the factors, as well as the prediction models between the photoacoustic peak-to-peak values and the concentration gradients of glucose. In experiments, the captured time-resolved photoacoustic signals of glucose were all averaged in 512 times, which effectively overcame the interference of noises to the photoacoustic signals of glucose, and improved the accuracy and effectivity of experimental data. Experimental results and the prediction results of models showed that compared with the wavelengths at 1 200 and 1 300 nm, the prediction model’s effect between the photoacoustic peak-to-peak values and the concentration of glucose at the wavelength of 1 064 nm was the best because its correlation coefficient of model was 0.986, which demonstrated that the glucose molecular had good absorption at characteristic wavelength of 1 064 nm. The photoacoustic peak-to-peak values of glucose linearly increased with the laser output energy, and the concentration prediction accuracy of glucose improved with the increase of the laser output energy. According to different concentration prediction results obtained by the ultrasonic transducers with different detection frequencies, compared with the ultrasonic transducers with frequency of 2.5 and 10 MHz, the photoacoustic detection effect of the glucose was the best for the ultrasonic transducer with frequency of 1 MHz. Finally, it was experimentally found that the photoacoustic peak-to-peak values of glucose linearly increased with the increasing of the temperatures and the concentrations. Especially, the concentration prediction error of glucose gradually increased with the increase of temperature.

任重, 刘国栋, 丁宇, 姚清凯. 基于OPO脉冲激发的血糖光声检测影响因素研究[J]. 光谱学与光谱分析, 2018, 38(10): 3023. REN Zhong, LIU Guo-dong, DING Yu, YAO Qing-kai. Studies on the Influence Factors of the Blood Glucose Photoacoustic Measurement Based on OPO Pulsed Laser Induction[J]. Spectroscopy and Spectral Analysis, 2018, 38(10): 3023.

关于本站 Cookie 的使用提示

中国光学期刊网使用基于 cookie 的技术来更好地为您提供各项服务,点击此处了解我们的隐私策略。 如您需继续使用本网站,请您授权我们使用本地 cookie 来保存部分信息。
全站搜索
您最值得信赖的光电行业旗舰网络服务平台!