为了提高壳聚糖的水溶性及其止血方面的性能, 将壳聚糖(CS)纳米化, 并引入具有抗菌作用的Ag+离子和凝血辅助作用的Ca2+离子, 制备出纳米壳聚糖金属离子复合止血材料。首先, 采用离子交换法制备纳米壳聚糖(nmCS), 再分别加入AgNO3和饱和CaCl2溶液, 制得nmCS-Ag、nmCS-Ca、nmCS-Ag-Ca复合材料。然后, 采用 FTIR、XRD、SEM等手段对复合材料的结构进行表征。最后, 对复合物的凝血、止血性能进行了测试。实验结果表明: 改性后的壳聚糖IR图谱在1 647 cm-1和1 560 cm-1处出现了纳米壳聚糖钠盐的特征吸收峰; 复合了金属离子的纳米壳聚糖在XRD图谱中表现出了Ag+、Ca2+的晶型特征; 扫描电镜显示nmCS-Ag中Ag+有部分析出而nmCS-Ca的复合效果较好; nmCS-Ag-Ca的凝血、止血效果要优于nmCS-Ag和nmCS-Ca, 同时nmCS-Ag和nmCS-Ca的凝血、止血效果要优于nmCS。测试结果表明, 成功制备了纳米壳聚糖金属离子复合止血材料。

In order to improve the water soluble and the hemostatic performance of chitosan(CS), chitosan is nano-sized and an antibacterial Ag+ and coagulation-assisted Ca2+ are introduced to prepare a nano-chitosan metal ion composite hemostatic material. Firstly, nano-chitosan(nmCS) is prepared by ion exchange method, and AgNO3 and saturated CaCl2 solution are added respectively to obtain nmCS-Ag, nmCS-Ca and nmCS-Ag-Ca composites. Then, the structure of the composite is characterized by means of FTIR, XRD and SEM. Finally, the coagulation and hemostatic properties of the complex are tested. The results show that the characteristic absorption peak of nano-chitosan sodium salt appeared at the modified chitosan IR spectrum at 1 647 cm-1 and 1 560 cm-1. The nano-chitosan composited with metal ions show the crystal characteristics of Ag+ and Ca2+ in the XRD pattern. The SEM shows that partial Ag+ precipitates in nmCS-Ag, and the composite effect of nmCS-Ca is better. The coagulation and hemostasis of nmCS-Ag-Ca is better than that of nmCS-Ag and nmCS-Ca, while the coagulation and hemostasis of nmCS-Ag and nmCS-Ca are better than that of nmCS. The test results show that the nano-chitosan metal ion composite hemostatic material has been successfully prepared.