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Intravital imaging of adriamycin-induced renal pathology using two-photon microscopy and optical coherence tomography

Intravital imaging of adriamycin-induced renal pathology using two-photon microscopy and optical coherence tomography

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Abstract

Adriamycin (doxorubicin), a common cancer chemotherapeutic drug, can be used to induce a model of chronic progressive glomerular disease in rodents. In our studies, we evaluated renal changes in a rat model after Adriamycin injection using two-photon microscopy (TPM), optical coherence tomography (OCT) and Doppler OCT (DOCT). Taking advantage of deep penetration and fast scanning speed for three-dimensional (3D) label-free imaging, OCT/DOCT system was able to reveal glomerular and tubular pathology noninvasively and in real time. By imaging renal pathology following the infusion of fluorophore-labeled dextrans of different molecular weights, TPM can provide direct views of glomerular and tubular flow dynamics with the onset and progression of renal disease. Specifically, glomerular permeability and filtration, proximal and distal tubular flow dynamics can be revealed. 6–8 weeks after injection of Adriamycin, TPM and OCT/DOCT imaging revealed glomerular sclerosis, compromised flow across the glomerular wall, tubular atrophy, tubular dilation, and variable intra-tubular flow dynamics. Our results indicate that TPM and OCT/DOCT provide real-time imaging of renal pathology in vivo that has not been previously available using conventional microscopic procedures.

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DOI:10.1142/s179354581850030x

基金项目:This work is supported by the National Institutes of Health (NIH) Grant Nos. R21AG042700 and R21DK088066. We thank Thomas W. Castonguay, Lily Jin, Zach Langley, and Phillip Liu for technical assistance.

收稿日期:2018-04-03

修改稿日期:2018-07-12

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Hengchang Guo:Fischell Department of Bioengineering University of Maryland College Park, MD 20742, USA
Hsing-Wen Wang:Fischell Department of Bioengineering University of Maryland College Park, MD 20742, USA
Qinggong Tang:Fischell Department of Bioengineering University of Maryland College Park, MD 20742, USA
Erik Anderson:Department of Biochemistry and Molecular & Cellular Biology Georgetown University Medical Center Washington DC 20007, USA
Reuben Falola:Department of Biochemistry and Molecular & Cellular Biology Georgetown University Medical Center Washington DC 20007, USA
Tikina Smith:Central Animal Resources Facility University of Maryland, College Park, MD 20742, USA
Yi Liu:Fischell Department of Bioengineering University of Maryland College Park, MD 20742, USA
Moshe Levi:Department of Biochemistry and Molecular & Cellular Biology Georgetown University Medical Center Washington DC 20007, USA
Peter M. Andrews:Department of Biochemistry and Molecular & Cellular Biology Georgetown University Medical Center Washington DC 20007, USA
Yu Chen:Fischell Department of Bioengineering University of Maryland College Park, MD 20742, USA

联系人作者:Yu Chen(yuchen@umd.edu)

【1】A. S. Go, G. M. Chertow, D. J. Fan, C. E. McCulloch, C. Y. Hsu, "Chronic kidney disease and the risks of death, cardiovascular events, and hospitalization," N. Engl. J. Med. 351, 1296–1305 (2004).

【2】A. S. Levey et al., "National kidney foundation practice guidelines for chronic kidney disease: Evaluation, classification, and stratification," Ann. Int. Med. 139, 137–147 (2003).

【3】R. J. Glassock, D. G. Oreopoulos, "Aging and chronic kidney disease," Nephron Clin. Pract. 119, c1–c1 (2011).

【4】M. Tonelli et al., "Chronic kidney disease and mortality risk: A systematic review," J. Am. Soc. Nephrol. 17, 2034–2047 (2006).

【5】A. S. Levey, J. Coresh, "Chronic kidney disease," The lancet 379, 165–180 (2012).

【6】A. S. Levey et al., "Definition and classification of chronic kidney disease: A position statement from Kidney Disease: Improving Global Outcomes (KDIGO)," Kidney Int. 67, 2089–2100 (2005).

【7】A. Chen et al., "Experimental focal segmental glomerulosclerosis in mice," Nephron 78, 440–452 (1998).

【8】E. Bucciarelli, R. Binazzi, P. Santori, G. Vespasiani, "Nephrotic syndrome in rats due to adriamycin chlorhydrate," Lav. Ist. Anat. Istol. Patol. Univ. Studi Perugia 36, 52–69 (1976).

【9】J. J. Nikken, G. P. Krestin, "MRI of the kidneystate of the art," Eur. Radiol. 17, 2780–2793 (2007).

【10】P. Andrews et al., "Optical coherence tomography of the living human kidney," J. Innov. Opt. Health Sci. 7, 1350064 (2014).

【11】P. M. Andrews et al., "High-resolution optical coherence tomography imaging of the living kidney," Lab. Invest. 88, 441–449 (2008).

【12】Y. Chen, P. M. Andrews, A. D. Aguirre, J. M. Schmitt, J. G. Fujimoto, "High-resolution three-dimensional optical coherence tomography imaging of kidney microanatomy ex vivo," J. Biomed. Opt. 12, 034008 (2007).

【13】J. Wierwille et al., "In vivo, label-free, threedimensional quantitative imaging of kidney microcirculation using Doppler optical coherence tomography," Lab. Invest. 91, 1596–1604 (2011).

【14】K. W. Dunn et al., "Functional studies of the kidney of living animals using multicolor two-photon microscopy," Am. J. Physiol. Cell Physiol. 283, C905–C916 (2002).

【15】S. L. Ashworth, R. M. Sandoval, G. A. Tanner, B. A. Molitoris, "Two-photon microscopy: Visualization of kidney dynamics," Kidney Int. 72, 416–421 (2007).

【16】C. L. Phillips et al., "Three-dimensional imaging of embryonic mouse kidney by two-photon microscopy," Am. J. Pathol. 158, 49–55 (2001).

【17】P. M. Andrews, W. M. Petroll, H. D. Cavanagh, J. V. Jester, "Tandem Scanning Confocal Microscopy (Tscm) of normal and ischemic living kidneys," Am. J. Anat. 191, 95–102 (1991).

【18】P. M. Andrews, B. S. Khirabadi, B. C. Bengs, "Using tandem scanning confocal microscopy to predict the status of donor kidneys," Nephron 91, 148–155 (2002).

【19】S. Quentmeier, S. Denicke, K. H. Gericke, "Twocolor two-photon fluorescence laser scanning microscopy," J. Fluores. 19, 1037–1043 (2009).

【20】P. T. C. So, H. Kim, I. E. Kochevar, "Two-photon deep tissue ex vivo imaging of mouse dermal and subcutaneous structures," Opt. Express 3, 339–350 (1998).

【21】W. Denk, J. H. Strickler, W. W. Webb, "Twophoton laser scanning fluorescence microscopy," Science 248, 73–6 (1990).

【22】H. C. Guo et al., "Two-photon fluorescence imaging of intracellular hydrogen peroxide with chemoselective fluorescent probes," J. Biomed. Opt. 18, 106002 (2013).

【23】W. R. Zipfel, R. M. Williams, W. W. Webb, "Nonlinear magic: Multiphoton microscopy in the biosciences," Nature Biotechnol. 21, 1368–1376 (2003).

【24】Y. Chen et al., "Recent advances in two-photon imaging: Technology developments and biomedical applications," Chin. Opt. Lett. 11, 011703 (2013).

【25】G. McConnell, E. Riis, "Two-photon laser scanning fluorescence microscopy using photonic crystal fiber," J. Biomed. Opt. 9, 922–927 (2004).

【26】F. Helmchen, W. Denk, "Deep tissue twophoton microscopy," Nature Meth. 2, 932–940 (2005).

【27】E. M. C. Hillman et al., "Depth-resolved optical imaging and microscopy of vascular compartment dynamics during somatosensory stimulation," Neuroimage 35, 89–104 (2007).

【28】M. B. Ericson et al., "Two-photon laser-scanning fluorescence microscopy applied for studies of human skin," J. Biophotonics 1, 320–330 (2008).

【29】D. G. Ouzounov et al., "In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain," Nature meth. 14, 388–390 (2017).

【30】W. Liang, G. Hall, B. Messerschmidt, M.-J. Li, X. Li, "Nonlinear optical endomicroscopy for label-free functional histology in vivo," Light: Science & Applications 6, e17082 (2017). DOI: 10.1038/lsa.2017.82.

【31】H. Koepsell, "In vivo two-photon fluorescence microscopy opens a new area for investigation of the excretion of cationic drugs in the kidney," Kidney Int. 72, 387–388 (2007).

【32】J. J. Kang, I. Toma, A. Sipos, F. McCulloch, J. Peti- Peterdi, "Quantitative imaging of basic functions in renal (patho)physiology," Am. J. Physiol. Renal Physiol. 291, F495–F502 (2006).

【33】B. A. Molitoris, R. M. Sandoval, "Intravital multiphoton microscopy of dynamic renal processes," Am. J. Physiol. Renal Physiol. 288, F1084–F1089 (2005).

【34】D. Huang et al., "Optical coherence tomography," Science 254, 1178–81 (1991).

【35】J. G. Fujimoto et al., "Optical biopsy and imaging using optical coherence tomography," Nature Med. 1, 970–972 (1995).

【36】G. J. Tearney et al., "In vivo endoscopic optical biopsy with optical coherence tomography," Science 276, 2037–2039 (1997).

【37】B. E. Bouma, S. H. Yun, B. J. Vakoc, M. J. Suter, G. J. Tearney, "Fourier-domain optical coherence tomography: Recent advances toward clinical utility," Curr. Opin. Biotechnol. 20, 111–118 (2009).

【38】C. Kut et al., "Detection of human brain cancer infiltration ex vivo and in vivo using quantitative optical coherence tomography," Sci. Trans. Med. 7, 292ra100 (2015).

【39】J. G. Fujimoto, C. Pitris, S. A. Boppart, M. E. Brezinski, "Optical coherence tomography: An emerging technology for biomedical imaging and optical biopsy," Neoplasia 2, 9–25 (2000).

【40】J. G. Fujimoto, "Optical coherence tomography for ultrahigh resolution in vivo imaging," Nat. Biotechnol. 21, 1361–1367 (2003).

【41】Z. P. Chen et al., "Noninvasive imaging of in vivo blood flow velocity using optical Doppler tomography," Opt. Lett. 22, 1119–1121 (1997).

【42】J. A. Izatt, M. D. Kulkami, S. Yazdanfar, J. K. Barton, A. J. Welch, "In vivo bidirectional color Doppler flow imaging of picoliter blood volumes using optical coherence tomograghy," Opt. Lett. 22, 1439–1441 (1997).

【43】Y. H. Zhao et al., "Doppler standard deviation imaging for clinical monitoring of in vivo human skin blood flow," Opt. Lett. 25, 1358–1360 (2000).

【44】R. K. Wang, "Optical microangiography: A labelfree 3-D imaging technology to visualize and quantify blood circulations within tissue beds in vivo," IEEE J. Sel. Top. Quantum Electron. 16, 545–554 (2010).

【45】Q. Zhang et al., "Wide-field optical coherence tomography based microangiography for retinal imaging," Sci. Rep. 6, 22017 (2016).

【46】V. W. S. Lee, D. C. H. Harris, "Adriamycin nephropathy: A model of focal segmental glomerulosclerosis," Nephrology 16, 30–38 (2011).

【47】J. W. Pippin et al., "Inducible rodent models of acquired podocyte diseases," Am. J. Physiol. Renal Physiol. 296, F213–F229 (2009).

【48】T. Bertani et al., "Adriamycin-induced nephrotic syndrome in rats — sequence of pathologic events," Lab. Invest. 46, 16–23 (1982).

【49】T. Bertani, F. Cutillo, C. Zoja, M. Broggini, G. Remuzzi, "Tubulointerstitial lesions mediate renal damage in adriamycin glomerulopathy," Kidney Int. 30, 488–496 (1986).

【50】J. Grond, J. J. Weening, J. D. Elema, "Glomerular sclerosis in nephrotic rats — comparison of the longterm effects of adriamycin and aminonucleoside," Lab. Invest. 51, 277–285 (1984).

【51】S. Okuda et al., "Adriamycin-induced nephropathy as a model of chronic progressive glomerular-disease," Kidney Int. 29, 502–510 (1986).

【52】H. Hackbarth et al., "Distribution of glomeruli in the renal cortex of munich wistar fromter (Mwf) rats," Renal Physiol. Biochem. 6, 63–71 (1983).

【53】Q. Tang et al., "Depth-resolved imaging of colon tumor using optical coherence tomography and fluorescence laminar optical tomography," Biomed. Opt. Exp. 7, 5218–5232 (2016).

【54】Q. Tang, C.-P. Liang, K. Wu, A. Sandler, Y. Chen, "Real-time epidural anesthesia guidance using optical coherence tomography needle probe," Quant. Imag. Med. Surg. 5, 118–124 (2014).

【55】Q. Tang et al., "High-dynamic-range fluorescence laminar optical tomography (HDR-FLOT)," Biomed. Opt. Exp. 8, 2124–2137 (2017).

【56】V. X. Yang et al., "High speed, wide velocity dynamic range Doppler optical coherence tomography (Part III): In vivo endoscopic imaging of blood flow in the rat and human gastrointestinal tracts," Opt. Exp. 11, 2416–2424 (2003).

【57】Q. Tang et al., "Real-time monitoring of microdistribution of antibody-photon absorber conjugates during photoimmunotherapy in vivo," J. Control. Release 260, 154–163 (2017).

【58】Z. Ding et al., "In Oxygen transport to tissue XXXVIII," (Eds. Q. Luo, L. Z. Li, D. K. Harrison, H. Shi, D. F. Bruley) 345–350, Springer International Publishing, Cham, (2016).

【59】H.-W. W. Bohan Wang, H. Guo, E. Anderson, Q. Tang, P. M. Andrews, Y. Chen, "Optical Coherence Tomography (OCT) and Computer-Aided Diagnosis (CAD) of a murine model of Chronic Kidney Disease (CKD)," J. Biomed. Opt. 22, 121706 (2017). DOI: 10.1117/1.JBO.22.12.121706.

【60】T. Kotyk et al., "Measurement of glomerulus diameter and Bowman''s space width of renal albino rats," Comput. Meth. Program. Biomed. 126, 143– 153 (2016).

【61】https://www.niddk.nih.gov/health-information/ kidney-disease/kidneys-how-they-work.

【62】J. Wartiovaara et al., "Nephrin strands contribute to a porous slit diaphragm scaffold as revealed by electron tomography," J. Clin. Invest. 114, 1475–1483 (2004).

【63】Y. S. Kanwar, Z. Z. Liu, N. Kashihara, E. I. Wallner, "Current status of the structural and functional basis of glomerular filtration and proteinuria," Semin. Nephrol. 11, 390–413 (1991).

【64】P. Mundel, S. J. Shankland, "Podocyte biology and response to injury," J. Am. Soc. Nephrol. 13, 3005– 3015 (2002).

【65】K. Endlich, W. Kriz, R. Witzgall, "Update in podocyte biology," Curr. Opin. Nephrol. Hypertens. 10, 331–340 (2001).

【66】K. Tryggvason, J. Wartiovaara, "Molecular basis of glomerular permselectivity," Curr. Opin. Nephrol. Hypertens. 10, 543–549 (2001).

【67】R. Rodewald, M. J. Karnovsky, "Porous substructure of the glomerular slit diaphragm in the rat and mouse," J. Cell Biol. 60, 423–433 (1974).

【68】H. P. Erickson, "Size and shape of protein molecules at the nanometer level determined by sedimentation, gel filtration, and electron microscopy," Biol. Proced. Online 11, 32–51 (2009).

【69】J. Peti-Peterdi, "Multiphoton imaging of renal tissues in vitro," Am. J. Physiol. Renal Physiol. 288, F1079–F1083 (2005).

【70】J. C. Jung, A. D. Mehta, E. Aksay, R. Stepnoski, M. J. Schnitzer, "In vivo mammalian brain Imaging using one- and two-photon fluorescence microendoscopy," J. Neurophysiol. 92, 3121–3133 (2004).

【71】J. Peti-Peterdi, I. Toma, A. Sipos, S. L. Vargas, "Multiphoton imaging of renal regulatory mechanisms," Physiol. 24, 88–96 (2009).

【72】A. H. Salmon et al., "Evidence for restriction of fluid and solute movement across the glomerular capillary wall by the subpodocyte space," Am. J. Physiol. Renal Physiol. 293, F1777–F1786 (2007).

【73】J. Peti-Peterdi, J. J. Kang, I. Toma, "Activation of the renal renin-angiotensin system in diabetes—new concepts," Nephrology Dialysis Transplantation 23, 3047–3049 (2008)

【74】K. W. Dunn, R. M. Sandoval, B. A. Molitoris, "Intravital imaging of the kidney using multiparameter multiphoton microscopy," Nephron Exp. Nephrol. 94, e7–e11 (2003).

【75】M. L. Onozato et al., "Optical coherence tomography of human kidney," J. Urol. 183, 2090–2094 (2010).

引用该论文

Hengchang Guo,Hsing-Wen Wang,Qinggong Tang,Erik Anderson,Reuben Falola,Tikina Smith,Yi Liu,Moshe Levi,Peter M. Andrews,Yu Chen. Intravital imaging of adriamycin-induced renal pathology using two-photon microscopy and optical coherence tomography[J]. Journal of Innovative Optical Health Sciences, 2018, 11(5): 1850030

Hengchang Guo,Hsing-Wen Wang,Qinggong Tang,Erik Anderson,Reuben Falola,Tikina Smith,Yi Liu,Moshe Levi,Peter M. Andrews,Yu Chen. Intravital imaging of adriamycin-induced renal pathology using two-photon microscopy and optical coherence tomography[J]. Journal of Innovative Optical Health Sciences, 2018, 11(5): 1850030

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