光子学报, 2018, 47 (11): 1128001, 网络出版: 2018-12-17
高灵敏磁分离荧光传感法检测黄曲酶毒素B1
Magnetic Separation Fluorescent Sensor for Highly Sensitive Detection of Aflatoxin B1
荧光传感器 痕量分析 黄曲霉毒素B1测定 磁性分离 核酸核酸适体 氨基-碳量子点 Fluorescent sensor Trace analysis Detection of aflatoxin B1 Magnetic separation Aptamers Aminofunctioned carbon quantum dots
摘要
以自组装方式制备了Au@Fe3O4/核酸适体/氨基-碳量子点磁性生物纳米复合物, 并提出一种磁分离荧光传感法用于黄曲霉毒素B1的检测.当样品中有黄曲霉毒素B1时, 磁性生物纳米复合物中核酸适体选择性地与黄曲霉毒素B1结合并释放出氨基-碳量子点, 经磁性分离后, 氨基-碳量子点留在溶液中, 体系溶液荧光强度随黄曲霉毒素B1浓度的增大而增强.黄曲霉毒素B1浓度在0.001~1.0 ng/mL范围与溶液荧光强度成良好线性关系, 线性相关系数为0.996 4, 检测限为0.3 pg/mL.该方法利用磁性分离技术, 有效地消除了背景荧光影响, 改善了荧光传感性能.
Abstract
A fluorescent aptasensor was developed and applied to detect aflatoxin B1 based on Au@Fe3O4/aptamers/aminofunctioned carbon quantum dots magnetic nanocomposites which were constructed self-assembly. The selective interactions between aflatoxin B1 and the aptamer in the samples cause release of the aminofunctioned carbon quantum dots which remains in the solution after magnetic separation. A linear fluorescence signal response to aflatoxin B1 concentration is obtained over a wide aflatoxin B1 concentration range of 0.001~1.00 ng/mL with a detection limit of 0.3 pg/mL, and the correlation coefficient is 0.996 4. The performances of the fluorescent sensor are significantly improved as the background fluorescence is effectively removed by magnetic separation.
曾云龙, 张敏, 易守军, 何盼, 赵敏, 夏晓东, 唐春然. 高灵敏磁分离荧光传感法检测黄曲酶毒素B1[J]. 光子学报, 2018, 47(11): 1128001. ZENG Yun-long, ZHANG Min, YI Shou-jun, HE Pan, ZHAO Min, XIA Xiao-dong, TANG Chun-ran. Magnetic Separation Fluorescent Sensor for Highly Sensitive Detection of Aflatoxin B1[J]. ACTA PHOTONICA SINICA, 2018, 47(11): 1128001.