We propose a novel optical method for glucose measurement based on diffuse photon-pair density wave (DPPDW) in a multiple scattering medium (MSM) where the light scattering of photon-pair is induced by refractive index mismatch between scatters and phantom solution. Experi-mentally, the DPPDW propagates in MSM via a two-frequency laser (TFL) beam wherein highly correlated pairs of linear polarized photons are generated. The reduced scattering coefficient 0 2s and absorption coefficient 2a of DPPDW are measured simultaneously in terms of the amplitude and phase measurements of the detected heterodyne signal under arrangement at different dis-tances between the source and detection fibers in MSM. The results show that the sensitivity of glucose detection via glucose-induced change of reduced scattering coefficient ( 0 2s) is 0.049% mM 1 in a 1% intralipid solution. In addition, the linear range of 0 2s vs glucose concentration implies that this DPPDW method can be used to monitor glucose concentration continuously and noninvasively subcutaneously.