Journal of Innovative Optical Health Sciences, 2017, 10 (2): 1641004, Published Online: Dec. 27, 2018  

Effect of spatial spectrum overlap on Fourier ptychographic microscopy

Author Affiliations
1 State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou 310027, P. R. China
2 Collaborative Innovation Center of Extreme Optics, Shanxi University, Taiyuan, 030006, P. R. China
Abstract
Fourier ptychographic microscopy (FPM) is a newly developed imaging technique which stands out by virtue of its high-resolution and wide FOV. It improves a microscope's imaging perfor-mance beyond the diffraction limit of the employed optical components by illuminating the sample with oblique waves of different incident angles, similar to the concept of synthetic aperture. We propose to use an objective lens with high-NA to generate oblique illuminating waves in FPM. We demonstrate utilizing an objective lens with higher NA to illuminate the sample leads to better resolution by simulations, in which a resolution of 0.28 m is achieved by using a high-NA illuminating objective lens (NA=1.49) and a low-NA collecting objective lens (NA=0.2) in coherent imaging (λ=488 nm). We then deeply study FPM's exact relevance of convergence speed to spatial spectrum overlap in frequency domain. The simulation results show that an overlap of about 60% is the optimal choice to acquire a high-quality recovery (520*520 pixels) with about 2 min's computing time. In addition, we testify the robustness of the algorithm of FPM to additive noises and its suitability for phase objects, which further proves FPM's potential application in biomedical imaging.

Qiulan Liu, Cuifang Kuang, Yue Fang, Peng Xiu, Yicheng Li, Ruixin Wen, Xu Liu. Effect of spatial spectrum overlap on Fourier ptychographic microscopy[J]. Journal of Innovative Optical Health Sciences, 2017, 10(2): 1641004.

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