首页 > 论文 > Journal of Innovative Optical Health Sciences > 10卷 > 5期(pp:1743004--1)

In vivo auditory nerve stimulation with visible light

In vivo auditory nerve stimulation with visible light

  • 摘要
  • 论文信息
  • 参考文献
  • 被引情况
  • PDF全文
分享:

Abstract

Background: Infrared laser stimulation has been proposed as an innovative method to elicit an auditory nerve response. Most studies have focused on using long-wavelength infrared (> 980 nm) pulsed lasers with high water absorption coe±cients. This paper sought to assess whether a shortwavelength laser (465 nm) with an absorption coe±cient as low as 10-3 cm-1 would activate the auditory nerve and studied its potential mechanism. Method: Optical compound action potentials (OCAPs) were recorded when synchronous trigger laser pulses stimulate the cochlea before and after deafening, varying the pulse durations (from 800 μs to 3600 μs) and the amount of radiant energy (from 18.05 mJ/cm2 to 107.91 mJ/cm2). A thermal infrared imager was applied to monitor the temperature change of the guinea pig cochlea. Results: The results showed that pulsed laser stimulation at 465nm could invoke OCAPs and had a similar waveform compared to the acoustical compound action potentials. The amplitude of OCAPs had a positive correlation with the increasing laser peak power, while the latency of OCAPs showed a negative correlation. The imager data showed that the temperature in the cochlea rose quickly by about 0.3C right after stimulating the cochlea and decreased quickly back to the initial temperature as the stimulation ended. Conclusions: This paper demonstrates that 465-nm laser stimulation can successfully induce OCAPs outside the cochlea, and that the amplitude and latency of the invoked OCAPs are highly affected by laser peak power. This paper proposes that a photothermal effect might be the main mechanism for the auditory nerve response induced by short-wavelength laser stimulation.

广告组1.2 - 空间光调制器+DMD
补充资料

DOI:10.1142/s1793545817430040

基金项目:This project was supported by grants from the Nature Science Foundation of China (Nos. NSFC8-1401539 and NSFC31271056), Shenzhen basic research layout project (JCYJ20160324163759208) and the projects in the Shenzhen Medical Engineering Laboratory For Human Auditoryequilibrium Function.

收稿日期:2017-07-15

修改稿日期:2017-08-10

网络出版日期:--

作者单位    点击查看

Tian Guan:Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, P. R. China
Muqun Yang:Tsinghua-Berkeley Shenzhen Institute, Tsinghua University, Shenzhen 518055, P. R. China
Yonghong He:Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, P. R. China

联系人作者:Tian Guan(guantian@sz.tsinghua.edu.cn)

【1】World Health Organization, “ Primary ear and hearing care training resource,” World Health Organization 2 (2006).

【2】G. M. Clark , “The multi-channel cochlear implant: Multi-disciplinary development of electrical stimulation of the cochlea and the resulting clinical benefit,” Hear. Res. 322, 4-13 (2015).

【3】L. G. Duckert, J. M. Miller , “Acute morphological changes in guinea pig cochlea following electrical stimulation: A preliminary scanning electron microscope study,” Ann. Otol. Rhinol. Laryngol. 91 (1), 33-40 (1982).

【4】S. Counter , “Electromagnetic stimulation of the auditory system: Effects and side-effects,” Scand. Audiol. Supp. 37, 1C32 (1993).

【5】A. I. Metric, J. T. Walsh, C. P. Richter , “Spatial extent of cochlear infrared neural stimulation determined by tone-on-light masking,” J. Biomed. Opt. 16 (11), 118002-1180028 (2011).

【6】A. D. Izzo, C. P. Richter, E. D. Jansen, J. T. Walsh , “Laser stimulation of the auditory nerve,” Lasers Surg. Med. 38 (8), 745-753 (2006).

【7】J. M. Bec, E. S. Albert, I. Marc et al., “Characteristics of laser stimulation by near infrared pulses of retinal and vestibular primary neurons,” Lasers Surg. Med. 44 (9), 736-745 (2012).

【8】R. L. Fork , “Laser stimulation of nerve cells in aplysia,” Science 171, 907-908 (1971).

【9】H. Hirase, V. Nikolenko, J. H. Goldberg, F. Yuste , “Multiphoton stimulation of neurons,” J. Neurobiol. 51, 237-247 (2002). Crossref,

【10】J. Wells, C. Kao, K. Mariappan, J. Albea, E. D. Jansen, P. Konrad, A. M. Jansen , “Optical stimulation of neural tissue in vivo,” Opt. Lett. 31, 235-238 (2005).

【11】A. Arvanitaki, N. Chalazonitis , “Excitatiory and inhibitory processes initiated by light and infrared radiations in single identifiable nerve cells,” Nervous Inhibition, Pergamon Press, New York (1961), pp. 194-231.

【12】J. Wells, C. Kao, E. D. Jansen et al., “Application of infrared light for in vivo neural stimulation,” J. Biomed. Opt. 10 (6), 064003 (2005).

【13】J. Wells, C. Kao, P. Konrad et al., “Biophysical mechanisms of transient optical stimulation of peripheral nerve,” Biophys. J. 93 (7), 2567-2580 (2007).

【14】A. D. Izzo, E. Suh, J. Pathria et al., “Selectivity of neural stimulation in the auditory system: A comparison of optic and electric stimuli,” J. Biomed. Opt. 12 (2), 021008-24000 (2007).

【15】A. D. Izzo, J. T. Walsh, E. D. Jansen et al., “Optical parameter variability in laser nerve stimulation: A study of pulse duration, repetition rate, and wavelength,” IEEE Trans. Biomed. Eng. 54 (6), 1108-1114 (2007).

【16】R. U. Verma, A. A. Guex, K. E. Hancock et al., “Auditory responses to electric and infrared neural stimulation of the rat cochlear nucleus,” Hear. Res. 310, 69-75 (2014).

【17】A. Fridberger, T. Ren , “Local mechanical stimulation of the hearing organ by laser irradiation,” Neuroreport 17 (1), 33-37 (2006).

【18】X. Tan, S. Rajguru, H. Young et al., “Radiant energy required for infrared neural stimulation,” Sci. Rep. 13273 (2015).

【19】H. K. Young, X. Tan, N. Xia et al., “Target structures for cochlear infrared neural stimulation,” Neurophotonics 2 (2), 025002 (2015).

【20】N. Kallweit, P. Baumhoff, A. Krueger et al., “Optoacoustic effect is responsible for laser-induced cochlear responses,” Sci. Rep. (2016).

【21】M. Schultz, P. Baumhoff, H. Maier et al., “Nanosecond laser pulse stimulation of the inner eara wavelength study,” Biomed. Opt. Express 3 (12), 3332-3345 (2012).

【22】N. Xia, X. Y. Wu, X. Wang et al., “Pulsed 808-nm infrared laser stimulation of the auditory nerve in guinea pig cochlea,” Lasers Med. Sci. 29 (1), 343-349 (2014).

【23】T. Guan, K. Zhu, F. Chen et al., “Auditory nerve impulses induced by 980 nm laser,” J. Biomed. Opt. 20 (8), 088004 (2015).

【24】A. D. Izzo, J. T. Walsh, H. Ralph et al., “Laser stimulation of auditory neurons: Effect of shorter pulse duration and penetration depth,” Biophys. J. 94 (8), 3159-3166 (2008).

【25】K. Scholle, P. Fuhrberg, P. Koopmann et al., “ 2m laser sources and their possible applications,” INTECH Open Access Publisher 10.5772/39538 (2010).

引用该论文

Tian Guan,Muqun Yang,Yonghong He. In vivo auditory nerve stimulation with visible light[J]. Journal of Innovative Optical Health Sciences, 2017, 10(5): 1743004

Tian Guan,Muqun Yang,Yonghong He. In vivo auditory nerve stimulation with visible light[J]. Journal of Innovative Optical Health Sciences, 2017, 10(5): 1743004

您的浏览器不支持PDF插件,请使用最新的(Chrome/Fire Fox等)浏览器.或者您还可以点击此处下载该论文PDF