Journal of Innovative Optical Health Sciences, 2017, 10 (5): 1743006, Published Online: Jan. 10, 2019  

Three-dimensional, isotropic imaging of mouse brain using multi-view deconvolution light sheet microscopy

Author Affiliations
1 School of Optical and Electronic Information, Huazhong University of Science and Technology, Wuhan 430074, P. R. China
2 Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, P. R. China
Abstract
We present a three-dimensional (3D) isotropic imaging of mouse brain using light-sheet fluorescent microscopy (LSFM) in conjunction with a multi-view imaging computation. Unlike common single view LSFM is used for mouse brain imaging, the brain tissue is 3D imaged under eight views in our study, by a home-built selective plane illumination microscopy (SPIM). An output image containing complete structural information as well as significantly improved resolution (~4 times) are then computed based on these eight views of data, using a bead-guided multi-view registration and deconvolution. With superior imaging quality, the astrocyte and pyramidal neurons together with their subcellular nerve fibers can be clearly visualized and segmented. With further including other computational methods, this study can be potentially scaled up to map the connectome of whole mouse brain with a simple light-sheet microscope.

Sa Liu, Jun Nie, Yusha Li, Tingting Yu, Dan Zhu, Peng Fei. Three-dimensional, isotropic imaging of mouse brain using multi-view deconvolution light sheet microscopy[J]. Journal of Innovative Optical Health Sciences, 2017, 10(5): 1743006.

关于本站 Cookie 的使用提示

中国光学期刊网使用基于 cookie 的技术来更好地为您提供各项服务,点击此处了解我们的隐私策略。 如您需继续使用本网站,请您授权我们使用本地 cookie 来保存部分信息。
全站搜索
您最值得信赖的光电行业旗舰网络服务平台!