原卟啉(PPIX)是血清中内源性荧光物质之一,目前研究表明癌症病人血液中的原卟啉含量高于正常人水平。因此,测定血清样品中的PPIX含量对于癌症病人的早期诊断具有重要的意义。本文中以超灵敏的荧光相关光谱(FCS)单分子检测技术,建立了直接测定血清中PPIX的含量的新方法。通过对100例正常人血清样本和12例疑似癌症病人血清样本(甲胎蛋白AFP含量异常)中PPIX的FCS数据分析,发现相对于检测PPIX-HSA的单个分子荧光强度和总荧光强度,PPIX-HSA在病人血清中的浓度与正常人血清有着极显著差异。FCS方法具有样品需求量少、分析时间短、可实现自动化操作等优点,有望成为癌症临床诊断的一种新方法。

Protoporphyrin(PPIX) is one of the endogenous chromophores in serum.Evidence suggests that PPIX in serum of cancer patients is higher than that of normal people.Therefore,direct detection of PPIX level in serum sample is of great value in the diagnosis of cancer at early stage.In this paper,we used fluorescence correlation spectroscopy(FCS) single molecule detection technology to directly detect the PPIX level in the serum.We applied FCS to directly detect PPIX level from serum samples of suspected cancer patients and healthy people.Comparing with the healthy people,the PPIX concentrations of suspected cancer patients were higher.The advantage of this method lies in its less sample requirement,shorter detection time,the posssibilityof automation.We believe that FCS method exhibits promising potential applications in the diagnosis of cancer at early stage.