Fluorescence tomography can obtain a sufficient dataset and optimal three-dimensional images when projections are captured over 360? by CCD camera. Herein a non-stop dynamic sampling mode for fluorescence tomography is proposed in an attempt to improve the optical measurement speed of the traditional imaging system and stability of the object to be imaged. A series of simulations are carried out to evaluate the accuracy of dataset acquired from the dynamic sampling mode. Reconstruction with the corresponding data obtained in the dynamic-mode process is also performed with the phantom. The results demonstrate the feasibility of such an imaging mode when the angular velocity is set to the appropriate value, thus laying the foundation for real experiments to verify the superiority in performance of this new imaging mode over the traditional one.