Cellular metabolism arouses the changes of substance in extracellular physiological microenvironment, and the metabolic level reflects the physiological state of cells. This paper developed a novel microphysiometer automatic analysis instrument based on multiparameter cell-based biosensors for quick drug analysis. This study included the multiparameter cell-based biosensors, cell culture chamber, drug auto-injection detection and analysis. The analysis instrument was capable of real-time detection for the acidic product and other chemical parameters generated by the cellular metabolism in the micro-volume. Finally, the paper employs human breast cancer cell line MCF-7 and drug experiments to verify the performance of microphysiometer, and study effects of different drugs on cell metabolism. Further, the research explores drug analysis method of the multiparameter microphysiometer. The results showed that the cell-based microphysiometer system provides a utility platform for rapid, long-term and automatic cell physiological environment detection and drug analysis.