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DEEP-UV CONFOCAL FLUORESCENCE IMAGING AND SUPER-RESOLUTION OPTICAL MICROSCOPY OF BIOLOGICAL SAMPLES

DEEP-UV CONFOCAL FLUORESCENCE IMAGING AND SUPER-RESOLUTION OPTICAL MICROSCOPY OF BIOLOGICAL SAMPLES

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Abstract

A wide range of techniques has been developed to image biological samples at high spatial and temporal resolution. In this paper, we report recent results from deep-UV confocal fluorescence microscopy to image inherent emission from fluorophores such as tryptophan, and structured illumination microscopy (SIM) of biological materials. One motivation for developing deep-UV fluorescence imaging and SIM is to provide methods to complement our measurements in the emerging field of X-ray coherent diffractive imaging.

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DOI:10.1142/s1793545812500253

基金项目:We acknowledge the support of the Australian Research Council for the Center of Excellence for Coherent X-ray Science (CE0561787). We also acknowledge collaborations with the groups of Prof. M. Ryan (La Trobe University), Prof. L. Tilley (La Trobe University/University of Melbourne). We also acknowledge Peter Wichta and Niels Zijlstra for assistance in some of the deep-UV imaging.

收稿日期:2012-07-28

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TREVOR A. SMITH:School of Chemistry and ARC Centre of Excellence for Coherent X-ray Science, University of Melbourne Parkville 3010, Australia
LIISA M. HIRVONEN:School of Chemistry and ARC Centre of Excellence for Coherent X-ray Science, University of Melbourne Parkville 3010, Australia
CRAIG N. LINCOLN:School of Chemistry and ARC Centre of Excellence for Coherent X-ray Science, University of Melbourne Parkville 3010, Australia
XIAOTAO HAO:School of Chemistry and ARC Centre of Excellence for Coherent X-ray Science, University of Melbourne Parkville 3010, Australia

联系人作者:TREVOR A. SMITH(trevoras@unimelb.edu.au)

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【11】T. A. Smith, C. N. Lincoln, Determination of 2-Photon Absorption Cross-Section of Tryptophan, unpublished.

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引用该论文

TREVOR A. SMITH,LIISA M. HIRVONEN,CRAIG N. LINCOLN,XIAOTAO HAO. DEEP-UV CONFOCAL FLUORESCENCE IMAGING AND SUPER-RESOLUTION OPTICAL MICROSCOPY OF BIOLOGICAL SAMPLES[J]. Journal of Innovative Optical Health Sciences, 2012, 5(4): 1250025

TREVOR A. SMITH,LIISA M. HIRVONEN,CRAIG N. LINCOLN,XIAOTAO HAO. DEEP-UV CONFOCAL FLUORESCENCE IMAGING AND SUPER-RESOLUTION OPTICAL MICROSCOPY OF BIOLOGICAL SAMPLES[J]. Journal of Innovative Optical Health Sciences, 2012, 5(4): 1250025

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