光谱学与光谱分析, 2019, 39 (2): 559, 网络出版: 2019-03-06
一个镍(Ⅱ)配合物的合成、 晶体结构及其与鲱鱼精DNA/BSA作用的光谱学研究
Synthesis, Crystal Structure and DNA/BSA Interaction of a Ni(Ⅱ) Coordination Compound
摘要
合成了配合物［Ni(2,2′-biby)(H2O)4］·(Htba)2·5H2O单晶, 分子式中2,2′-biby为2,2′-联吡啶, H2tba为2-硫代巴比妥酸。 通过红外光谱、 元素分析和单晶X-射线衍射表征其结构, 单晶X-射线衍射结果表明, 该配合物单晶属于正交晶系, Pbca 空间群, 晶胞参数为a=13.662(2) , b=19.470(4) , c=21.590(4) 。 应用紫外光谱和荧光光谱研究了其与鲱鱼精DNA/牛血清白蛋白(BSA)的相互作用, 研究结果表明, 标题化合物与DNA为静电结合, 对牛血清白蛋白发生静态猝灭作用。
Abstract
A Ni(Ⅱ) coordination complex ［Ni(2,2′-biby) (H2O)4］·(tba)2·5H2O(2,2′-biby=2,2′-dipyridine, H2tba=2-thiobarbituric acid ) has been synthesized and structurally characterized by IR, element analysis and single crystal X-ray diffractometry. The results of single crystal X-ray diffractometry displayed that the title compound belongs to orthorhombic with space group of Pbca, and its cell parameters are a=13.662(2) , b=19.470(4) , c=21.590(4) . The binding of title compound with Herring sperm DNA/BSA has been investigated by absorption and fluorescence spectra, and the results displayed that the title compound binds with DNA in electrostatic interaction mode, and quenches the intrinsic fluorescence of BSA by a static quenching mechanism.
彭程佳, 王克华, 陆为学, 管纯倩, 胡雅静, 马双, 朱明昌, 高恩君. 一个镍(Ⅱ)配合物的合成、 晶体结构及其与鲱鱼精DNA/BSA作用的光谱学研究[J]. 光谱学与光谱分析, 2019, 39(2): 559. PENG Chen-jia, WANG Ke-hua, LU Wei-xue, GUAN Chun-qian, HU Ya-jing, MA Shuang, ZHU Ming-chang, GAO En-jun. Synthesis, Crystal Structure and DNA/BSA Interaction of a Ni(Ⅱ) Coordination Compound[J]. Spectroscopy and Spectral Analysis, 2019, 39(2): 559.
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