激光与光电子学进展, 2019, 56 (5): 051701, 网络出版: 2019-07-31
受细胞外液扰动的白细胞散射偏振特征 下载: 1122次
Scattering Polarization Characteristics of White Blood Cells Disturbed by Extracellular Fluid
生物光学 细胞分析 光散射 偏振 米勒矩阵 白细胞 biotechnology cell analysis light scattering polarization Mueller matrix white blood cells
摘要
针对白细胞受细胞外液扰动发生形变的情况,建立了无粒白细胞的椭球核切比雪夫形光学模型。基于T 矩阵方法,对线偏振光入射时形态参数及细胞核质比对白细胞散射偏振的影响进行了数值仿真,并与不受细胞外液扰动时的散射偏振特性进行了对比。研究结果表明,在侧向散射区,白细胞散射光线偏振度对外液扰动强度的变化相对于在后向散射区更为敏感,外液扰动越大对偏振的影响越大,且内核形变越大,外液扰动对白细胞散射光偏振的影响越大。
Abstract
An optical model of Chebyshev-shaped particle with ellipsoidal nucleus is established for agranulocyte, aiming at their morphological changes due to the disturbance of extracellular fluid. Based on the T-matrix method, the scattering characteristics of linear polarization of the white blood cells (WBCs) at different morphometric parameters and nucleus to radius ratios are numerically simulated for linearly polarized incident light. The scattering polarization characteristics are compared with those of WBCs without the disturbance of extracellular fluid. The research results show that the degree of linear polarization of the scattered light is more sensitive to the external liquid disturbance at the side-scattering region than that at the backscattering region, the stronger the external fluid disturbance, the greater the influence on light polarization. Moreover, the larger the nuclear deformation parameter, the greater the influence of extracellular fluid on the polarization of scattered light.
叶坤涛, 夏雪婷, 吉俄木沙. 受细胞外液扰动的白细胞散射偏振特征[J]. 激光与光电子学进展, 2019, 56(5): 051701. Kuntao Ye, Xueting Xia, Musha Ji'e. Scattering Polarization Characteristics of White Blood Cells Disturbed by Extracellular Fluid[J]. Laser & Optoelectronics Progress, 2019, 56(5): 051701.