利用红外光谱技术分析感染小斑病的玉米叶片,获取了染病玉米叶片的分子结构信息,研究结果表明真菌病原体对叶片中的蛋白质结构有一定的影响。二维相关红外光谱分析结果显示,健康玉米叶片中蛋白质二级结构的β-折叠构象随着玉米叶片的生长代谢发生变化,而染病玉米叶片中发生变化的是β-转角构象。二维相关红外光谱可以揭示真菌病原体入侵时玉米叶片分子结构的变化情况,为玉米病害的防治提供参考。

The infrared spectroscopy method is employed to analyze the corn leaves infected by the southern corn leaf blight disease and the molecular structure information of infected corn leaves is obtained. The research results show that the fungal pathogens have certain effects on the protein structure of the leaves. The two-dimensional correlation infrared spectral analysis indicates that the β-sheet conformation of the protein secondary structure in healthy corn leaves changes with the growth and metabolism of corn leaves, while in the infected corn leaves is the change of the β-turn conformation. Two-dimensional correlation infrared spectroscopy can be used to reveal the change of molecular structures of corn leaves invaded by the fungal pathogens and provide a reference for the prevention and control of corn diseases.