拉曼光谱是提供物质结信息的强有力工具。 但由于拉曼散射信号弱, 灵敏度低, 因此应用范围受到限制。 而在共振拉曼光谱(RRS)中, 由于激发光源频率落在分子的某一电子吸收带内, 分子吸收光子向电子激发态的跃迁变成了共振吸收, 因此对入射光的吸收强度大大增加。 与常规拉曼光谱相比, RRS能够提高信号强度的106倍。 因此, RRS检测技术以其更高的灵敏度和选择性而具有更广的应用, 特别是在生物学及医学等领域。 如： (1)生物基质中的类胡萝卜素和叶绿素等色素分析; (2)细胞、 蛋白质和DNA等有机物研究以及一些临床疾病诊断。 RRS可以得到在常规拉曼光谱中隐藏的、 更为重要的分子结构信息。 RRS总是在很低的浓度下测试, 且共振拉曼增强的谱线是属于产生电子吸收的基团, 这对于有色物和生物样品尤为重要。 因为很多这类样品的活性部位接近于生色基团, 且研究对象往往是生物大分子的某一部分, 所以在研究生物物质的结构和功能的关系时, RRS起着重要作用。 近年来, 由于光谱技术的发展使得RRS检测技术得到创新与延伸, 如液芯光纤共振拉曼光谱和透射共振拉曼光谱等新技术的应用。 通过对近几年有关RRS技术应用的原始论文、 数据和主要观点进行归纳整理与分析提炼, 介绍了RRS这一专题的历史背景和研究现状, 分别对共振拉曼光谱的色素检测、 生物检测和爆炸物检测等应用领域展开详细的综述, 并介绍了相关新技术的发展应用。 随着光谱技术的快速发展, RRS必将在科研领域拥有其他光谱技术不可取代的重要地位。

Raman spectroscopy is a powerful tool for providing information about material structure, but its application scope is limited due to its weak Raman scattering signal and low sensitivity. However, in resonant Raman spectroscopy(RRS), the absorption intensity of the incident light by the molecule is greatly increased due to the frequency of the excitation light source falls within one electron absorption band of the molecule and the transition to the electron excited state of molecule becomes a resonance absorption after absorbting photons. RRS can increase the signal intensity by a factor of 106 compared to conventional Raman spectroscopy. Therefore, it is more widely applied with its higher sensitivity and selectivity, especially in the fields of biology and medicine. For instance: (1) Analysis of pigments such as carotenoid and chlorophyll et al. in biological matrices; (2) Researches on organic substances such as cells, proteins, and DNA, as well as the diagnosis of some clinical diseases. RRS can obtain more important information of molecular structure which is hidden in normal Raman spectroscopy. RRS can be achieved at very low concentration, and the Raman lines with resonance Raman enhancement belong to the group that can generate electron absorption, which is crucial to coloured substance and biological samples. The active sites of many of these samples are close to the chromophore groups, and the object of research is often one part of biological macromolecules, so RRS plays an important role in researching the relationship of the structure and function of biological substances. In recent years, RRS has been innovated and extended such as the application of new technologies of Liquid-core optical fiber Resonance Raman spectroscopy and Transmission Resonance Raman spectroscopy with the development of spectroscopy. This view summarizes and analyzes the raw paper, data and main viewpoint of RRS technology applications in recent years. It introduced the historical background and research status of RRS, and carried out a detailed overview of the application of resonance Raman spectroscopy in the fields of pigment detection, biology detection and explosive detection as well as the development and application of relevant new technology. RRS will have an irreplaceable position in the field of scientific research field with the rapid development of spectroscopy technology.