傅里叶叠层显微术(Fourier ptychographic microscopy, FPM)是一极具前景的计算成像技术, 它具有高分辨率、大视场、无标记和定量相位等优势。由于它灵活的系统、高对比度的成像结果、无需干涉装置和光源机械扫描部件, 在数字病理学、体外细胞无标记观察和实时监测等方面得到了大量的研究和应用。文中主要介绍了FPM技术的系统误差校正方法、基于FPM的高通量显微成像和高速显微成像技术研究的基本原理、研究现状和最新进展, 提出了目前面临的问题以及未来的发展趋势。

Fourier ptychographic microscopy(FPM) is a promising label-free computational imaging technique with high resolution, wide field-of-view(FOV) and quantitative phase recovery. Due to its flexible setup, promising high-contrast performance without mechanical scanning and interferometric measurements, FPM has wide applications in the digital pathology, observation and dynamic imaging of label-free cells in vitro. In this review, the principle, research status and the latest advances were introduced in several aspects of FPM such as the system calibration methods, high-throughput imaging and high-speed imaging. The current problems and future trends were also presented.