为了实现光动力治疗过程中单线态氧的检测, 设计了一种水溶性良好的单线态氧纳米探针。首先利用再沉淀法将1,3二苯基异苯并呋喃(DPBF)封装于纳米颗粒中, 然后对纳米探针进行透射电镜、动态光散射粒径和吸收光谱表征, 表明纳米颗粒具有良好的分散性, 粒径约为200 nm, 在426 nm左右具有DPBF的特征吸收峰, 最后选择吲哚菁绿(ICG)作为光敏剂, 利用纳米探针的吸收峰变化检测单线态氧的产生。结果表明该纳米探针对单线态氧具有高灵敏度, 在光动力治疗过程中单线态氧的检测方面具有良好的应用价值。

In order to realize the detection of singlet oxygen during photodynamic therapy, a singlet oxygen nanoprobe with good water solubility was designed. Firstly, the 1,3 diphenylisobenzofuran(DPBF) was encapsulated in the nanoparticles by reprecipitation. Then the nano-probes were characterized by transmission electron microscopy, dynamic light scattering particle size and absorption spectrum. The nanoparticle with good dispersibility is about 200 nm and has a characteristic absorption peak of DPBF at around 426 nm. Finally, indocyanine green(ICG) was selected as a photosensitizer, and the generation of singlet oxygen was detected by the change of the absorption peak of the nanoprobe. The results show that the nanoprobe has high sensitivity to singlet oxygen and is promising in the monitoring of singlet oxygen for photodynamic therapy.