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Fluorescence life-time imaging microscopy (FLIM) monitors tumor cell death triggered by photothermal therapy with MoS2 nanosheets

Fluorescence life-time imaging microscopy (FLIM) monitors tumor cell death triggered by photothermal therapy with MoS2 nanosheets

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Abstract

Recently, photothermal therapy (PTT) has been proved to have great potential in tumor therapy. In the last several years, MoS2, as one novel member of nanomaterials, has been applied into PTT due to its excellent photothermal conversion e±cacy. In this work, we applied fluorescence lifetime imaging microscopy (FLIM) techniques into monitoring the PPTtriggered cell death under MoS2 nanosheet treatment. Two types of MoS2 nanosheets (single layer nanosheets and few layer nanosheets) were obtained, both of which exhibited presentable photothermal conversion e±cacy, leang to high cell death rates of 4T1 cells (mouse breast cancer cells) under PTT. Next, live cell images of 4T1 cells were obtained via directly labeling the mitochondria with Rodamine123, which were then continuously observed with FLIM technique. FLIM data showed that the fluorescence lifetimes of mitochondria targeting dye in cells treated with each type of MoS2 nanosheets significantly increased during PTT treatment. By contrast, the fluorescence lifetime of the same dye in control cells (without nanomaterials) remained constant after laser irradiation. These findings suggest that FLIM can be of great value in monitoring cell death process during PTT of cancer cells, which could provide dynamic data of the cellular microenvironment at single cell level in multiple biomedical applications.

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DOI:10.1142/s1793545819400029

基金项目:This work was supported by the National Key R&D Program of China (2018YFC0910602), the National Natural Science Foundation of China (Grant Nos. 31771584/61775145/61605121, 61620106016/61525503/61835009/81727804), Guangdong Natural Science Foundation Innovation Team (2014A030312008), and Shenzhen Basic Research Project (JCYJ20170818100153423/JCYJ201704 12110212234/JCYJ20160328144746940/JCYJ2017 0412105003520/JCYJ20170302142902581), Science Foundation of SZU (Grant No. 000193).

收稿日期:2019-03-14

修改稿日期:2019-05-06

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Hongda Liang:Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, P. R. China
Zheng Peng:Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, P. R. China
Xiao Peng:Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, P. R. China
Yufeng Yuan:Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, P. R. China
Teng Ma:Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, P. R. China
Yiwan Song:Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, P. R. China
Jun Song:Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, P. R. China
Junle Qu:Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, P. R. China

联系人作者:Xiao Peng(pengxiao_px@szu.edu.cn)

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引用该论文

Hongda Liang,Zheng Peng,Xiao Peng,Yufeng Yuan,Teng Ma,Yiwan Song,Jun Song,Junle Qu. Fluorescence life-time imaging microscopy (FLIM) monitors tumor cell death triggered by photothermal therapy with MoS2 nanosheets[J]. Journal of Innovative Optical Health Sciences, 2019, 12(5): 1940002

Hongda Liang,Zheng Peng,Xiao Peng,Yufeng Yuan,Teng Ma,Yiwan Song,Jun Song,Junle Qu. Fluorescence life-time imaging microscopy (FLIM) monitors tumor cell death triggered by photothermal therapy with MoS2 nanosheets[J]. Journal of Innovative Optical Health Sciences, 2019, 12(5): 1940002

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