应用激光, 2019, 39 (5): 909, 网络出版: 2019-12-05  

TBO-PDT介导光动力疗法抑菌作用对牙体及牙周的影响

Effects of TBO-PDT Mediated Photodynamic Therapy on the Bacteriostasis of Teeth and Periodontal Tissue
作者单位
1 河北省承德市承德医学院附属医院口腔科, 河北 承德 067000
2 河北省承德市承德医学院, 河北 承德 067000
摘要
目的:探讨以甲苯胺蓝O(toluidine blue O, TBO)为光敏剂的光动力疗法(photodynamic therapy, PDT)抑制大鼠混合菌生物膜内主要致龋菌作用的影响。方法:以变形链球菌、血链球菌、嗜酸性乳杆菌和粘性放线菌为实验菌株, 建立牙菌斑生物膜模型。实验分为5组, 将40只大鼠随机分配, 8只大鼠为一组:A组用生理盐水处理, 作为阴性对照; B组用洗必泰处理, 作为阳性对照组; C组单纯激光组, 选择波长为630 nm, 应用输出强度为105 mW/cm2, 照射时间为9 min。D组单纯光敏剂组, 光敏剂浓度100 mg/L避光孵育5 min。E组TBO-PDT组, 光敏剂浓度为100 mg/L然后避光孵育5 min后, 应用波长为630 nm, 输出强度为105 mW/cm2进行激光照射。每只大鼠口腔分为4个区, 均选择每区内最后2个磨牙, 每只大鼠共计8个样本牙。平板菌落计数观察牙菌斑生物膜活性, 组织病理切片观察PDT对实验动物口腔软组织的影响。扫描电镜观察PDT作用前后牙齿表面形态的变化。结果: 与生理盐水处理组, 洗必泰处理组相比, TBO-PDT处理组牙菌斑内致龋菌存活的数量( CFU/mL)明显减少(P<0.05),其抑菌率为89.07%; 而C组与D组无明显抑菌效果。扫描电镜显示TBO-PDT组的磨牙表面比较光滑, 脱矿孔表浅。病理切片示TBO-PDT组牙周组织无明显损伤。结论:实验表明光动力疗法有明显的防龋效果, 且对牙体硬组织及牙周组织无明显损伤。
Abstract
Object: To investigate the effect of photodynamic therapy with TBO as photosensitizer on inhibition of cariogenic bacteria in rat biofilm.Method:A model of dental plaque biofilm was established by using streptococcus mutans, streptococcus haematococcus, lactobacillus acidophilus and actinomyces glutinous as experimental strains. The experiment was divided into five groups. Forty rats were randomly divided into five groups. There were 8 rats in each group. Group A was the negative treatment group treated with normal saline; Group B was the positive control group treated chlorhexidine; Group C was treated with laser radiation only. The parameter was a wavelength of 630 nm, an output intensity of 105mW/cm2, irradiation time of 9 min; Group D was treated with photosensitizer only whose concentration was 100mg/L and incubated in dark for 5min; Group E was named TBO-PDT group. TBO-PDT group was treated with 100 mg/L TBO , incubated in dark for 5min and irradiated with diode laser which parameter was wavelength 630nm and output intensity 105mw/cm2.The oral cavity of each rat was divided into 4 regions. The last two molars in each area were selected as experimental objects. 8 sample teeth were selected for each rat.The activity of dental plaque biofilm was observed by plate colony counting experiment. The effect of PDT on oral soft tissue was observed by histopathological section. Scanning electron microscopy was used to observe the changes of tooth surface morphology before and after PDT treatment.Result: Compared with Group A and Group B, the number (CFU/mL) of viable caries causing bacteria in the dental plaque of Group E was significantly reduced (P<0.05). The bacteriostasis rate was 87.02%. Group C and Group D have no obvious bacteriostatic effect. Scanning electron microscopy showed that the molars of TBO-PDT group had smooth surface and superficial demineralization pore. Pathological section showed no obvious periodontal tissue damage in TBO-PDT group.Conclusion: The experimental results showed that photodynamic therapy had obvious anti-caries effects and had no obvious damage to the dental hard tissue and periodontal tissue.

陶亚东, 柳雪, 霍峰, 陈喜波, 王敬. TBO-PDT介导光动力疗法抑菌作用对牙体及牙周的影响[J]. 应用激光, 2019, 39(5): 909. Tao Yadong, Liu Xue, Huo Feng, Chen Xibo, Wang Jing. Effects of TBO-PDT Mediated Photodynamic Therapy on the Bacteriostasis of Teeth and Periodontal Tissue[J]. APPLIED LASER, 2019, 39(5): 909.

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