光学技术, 2019, 45 (5): 562, 网络出版: 2020-01-07
64通道单粒子荧光光谱数据采集系统
64-Channels single-particle fluorescence spectrum data acquisition system
激光诱导荧光 单粒子荧光 64通道PMT阵列 Laser Induced Fluorescence Single-particle fluorescence 64 channels PMT array
摘要
为了提高微弱荧光信号的探测能力, 获取高分辨光谱信息, 通过2套32通道光电倍增管(PMT)阵列, 结合激光诱导荧光技术, 搭建了64通道荧光光谱数据采集系统, 并使用LabVIEW对数据采集系统进行软件控制, 实现光谱数据的高速采集与处理。实验结果表明该采集系统光谱分辨率为4.69nm, 能够对微流控芯片内通过的单粒子样品溶液进行荧光光谱采集, 实现计数功能。
Abstract
In order to improve the detection ability of weak fluorescence signals, acquire high-spectral resolution information, a 64 channels of fluorescence spectral data acquisition system is built by combining two sets of 32-channel photomultiplier tube (PMT) arrays with laser-induced fluorescence technology, and LabVIEW is used for software control of data acquisition system. The acquisition system achieves the high-speed acquisition and processing of spectral data. The experimental results show that the system can perform the fluorescence spectra acquisition of the single-particle sample solution passing through the microfluidic chip and realize the counting function, and the spectral resolution of the acquisition system is 4.69nm.
张寅超, 姚娇娇, 杜宪炟, 陈和, 郭磐. 64通道单粒子荧光光谱数据采集系统[J]. 光学技术, 2019, 45(5): 562. ZHANG Yinchao, YAO Jiaojiao, DU Xianda, CHEN He, GUO Pan. 64-Channels single-particle fluorescence spectrum data acquisition system[J]. Optical Technique, 2019, 45(5): 562.