NAC转录因子, 对植物的生长发育及抵御逆境胁迫起着重要的作用。实验室前期克隆了辽宁碱蓬(Suaeda liaotungensis)Slnac2基因, 转Slnac2拟南芥(Arabidopsis thaliana)提高了抵御盐胁迫的能力。本研究利用基因芯片技术筛选转SlNAC2拟南芥差异表达基因, 共筛选出差异表达2倍以上的基因1 258个。GO富集度分析结果显示, 分子功能相关基因占34.06%, 细胞组分相关基因占33.13%, 生物学过程相关基因占32.81%。KEGG分析表明, 差异表达基因涉及63个信号通路, 主要有植物激素信号转导、核糖体、植物病原物相互作用和抗坏血酸代谢等。通过实时荧光定量PCR对部分差异基因进行验证, 所得结果与基因芯片结果一致。本研究揭示, SlNAC2可调控多个下游基因的表达, 提高植物在逆境胁迫下的生存能力。

NAC transcription factors play an important role in plant growth development and resistance to stress. In our previous studies, the Slnac2 gene was cloned from Suaeda liaotungensis and transformed into Arabidopsis. SlNAC2 enhanced the salt tolerance of transgenic Arabidopsis. In this study, we screened the differentially expressed genes in transgenic Slnac2 Arabidopsis compared to the wild type by cDNA microarray. There are 1 258 genes which expressed more than two-fold in transgenic Arabidopsis. The results of GO enrichment analysis showed that the molecular function-related genes accounted for 34.06%, the cell-related genes accounted for 33.13%, and the biological process-related genes accounted for 32.81%. KEGG(Kyoto Encyclopedia of Genes and Genomes pathways enrichment)analysis showed that 1 258 genes were involved in 63 different signaling pathways including plant hormone signal transduction, ribosome plant-pathogen interaction, ascorbate and aldarate metabolism, etc. Some differential genes were verified by real-time PCR, and the results were consistent with those of the gene chip. In summary, SlNAC2 can regulate the expression of numerous downstream genes and improve plant viability under stress.