缺磷已成为目前制约作物产量提高的非生物胁迫之一, 筛选作物耐低磷资源, 进行作物耐低磷育种已成为作物科学当前研究的热点之一, 因此植物体内磷含量的及时有效的监测也变得尤为重要。本文通过改良前后的钼锑抗比色法测定谷子新鲜幼嫩叶片和幼嫩根系的组织磷含量, 发现改良后的钼锑抗比色法所测的磷含量在一定范围内有良好的线性关系, 线性方程为y=1.060 3x-0.010 3, 线性相关系数达0.998 1。用该方法对谷子新鲜幼嫩叶片、幼嫩根系、成熟叶片、成熟根系、新鲜幼穗、成熟茎秆及液氮冷冻后的相应组织和谷子籽粒的磷含量以及不同磷浓度处理下(0.005、0.250 mmol/L)的谷子材料的磷含量进行测定, 进一步证实了其具有良好的灵敏度和精密度。该方法所测得的标准偏差较小, 在0~0.015 8范围之内; 相对标准偏差(RSD)的范围在0%~1.81%; 检出限的范围在0~0.047 4 μg/mL, 重复性较好,利用该方法还可有效地筛选具有磷吸收效率差异的谷子品种。改良后的钼锑抗比色法测定结果线性良好, 标准差低, 精密度很理想, 适合植物中低浓度磷的测定, 可为不同谷子材料总磷测定提供依据和参考。

Phosphorus deficiency has become one of the abiotic stresses that hinder the enhancement of crop yield. It has become one of the hot spots in crop science to screen out low phosphorus tolerance resources and carry out crop breeding for low phosphorus tolerance, and it is particularly important to monitor the phosphorus content in plants timely and effectively. In this paper, the content of phosphorus in the tissues of fresh and young leaves and roots of foxtail millet were determined by molybdenum antimony anti-colorimetric method before and after improvement. It was found that the phosphorus content measured by the modified molybdenum antimony anti-colorimetric method has a good linear relationship with the colorimetric absorbance in a certain range. The linear equation was y=1.060 3x-0.010 3, and the linear correlation coefficient was 0.998 1. Using the method for foxtail millet fresh tender leaf, mature young root, leaf, root, fresh young ear, mature stalks and those tissues which was frozen by liquid nitrogen, and the foxtail millet seeds, the phosphorus content from these different millet crop tissues, and their phosphorus content under different phosphorus concentration treatments (0.005, 0.250 mmol/L) were determined. It is proved that this method has good sensitivity and precision, and the standard deviation measured by this method is small, within the range of 0~0.015 8. The relative standard deviation (RSD) ranges from 0% to 1.81%. The detection limit ranges from 0 to 0.047 4 μg/mL, with good repeatability. The method can also be used to effectively screen foxtail millet varieties with different phosphorus absorption efficiency. Modified molybdenum-antimony anti-colorimetric method has agood linearity, low standard deviation and ideal precision, which is suitable for the determination of low concentration phosphorus in plants, and can provide a basis and reference for the determination of total phosphorus in different foxtail millet materials.