发光学报, 2020, 41 (9): 1045, 网络出版: 2020-11-02  

基于稀土发光纳米材料的时间分辨成像

Time-resolved Imaging Using Lanthanide-doped Nanomaterials
作者单位
哈尔滨工业大学 化工与化学学院, 黑龙江 哈尔滨 150001
摘要
时间分辨成像是一种在时域层面区分荧光信息的成像技术。该技术能有效地消除自体荧光和光散射的背景干扰,从而极大地提高成像信噪比和灵敏度; 同时该成像技术不依赖于生物组织的厚度,适用于生物活体多通道定量检测。稀土发光纳米材料具有独特的光学性质,不仅在生物窗口内存在多个近红外窄带辐射,而且具备长荧光寿命(微秒-毫秒范围,长于生物内源性荧光团3个数量级以上),适用于时间分辨生物成像。特别是稀土纳米晶可以通过调节纳米结构和组分实现其荧光寿命的人工精确调控,从而制备出系列寿命编码型探针应用于活体时间域分辨成像。本文主要阐述了稀土发光纳米材料荧光寿命的普适性调控策略,系统地综述了该材料在时间分辨成像中的最新研究进展,并展望了其未来发展趋势。
Abstract
Time-resolved imaging is an emerging type of imaging technique which can resolve long lifetime luminescence signal against short lifetime background signal in the time domain. The suppression of autofluorescence and scattering background endows this imaging technique with high signal-to-noise ratios and sensitivities. Lanthanide-doped nanomaterials have unique optical properties that make them suitable to serve as τ-dots for time-resolved imaging, such as being able to emit a multitude of narrow-band emissions in biological windows, being photostable under high laser irradiance, and having ultralong luminescence lifetimes in the μs-ms range about more than three orders of magnitude longer than the ones of biological endogenous fluorophores. Importantly, manipulation of the nanostructure and composition of lanthanide doped nanomaterials can result in a precise control over the luminescence lifetime, thus producing a set of lifetime-encoded biolabels for high-throughput multichannel detections. This review presents the general strategies utilized to regulate the luminescence lifetime of lanthanide-doped nanomaterials, highlights the most recent advances of utilizing them for time resolved imaging, and casts a look at their future developments.

王鑫, 韩晓军, 陈冠英. 基于稀土发光纳米材料的时间分辨成像[J]. 发光学报, 2020, 41(9): 1045. WANG Xin, HAN Xiao-jun, CHEN Guan-ying. Time-resolved Imaging Using Lanthanide-doped Nanomaterials[J]. Chinese Journal of Luminescence, 2020, 41(9): 1045.

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