Photonics Research, 2019, 7 (9): 09001042, Published Online: Aug. 13, 2019   

Simultaneous dual-contrast three-dimensional imaging in live cells via optical diffraction tomography and fluorescence Download: 638次

Author Affiliations
1 Nanophotonics Research Centre, Shenzhen Key Laboratory of Micro-Scale Optical Information Technology, Shenzhen University, Shenzhen 518060, China
2 Department of Chemistry and Physics, La Trobe Institute for Molecular Science (LIMS), La Trobe University, Melbourne, Victoria 3086, Australia
3 Department of Optics and Optical Engineering, Anhui Key Laboratory of Optoelectronic Science and Technology, University of Science and Technology of China, Hefei 230026, China
4 Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science (LIMS), La Trobe University, Melbourne, Victoria 3086, Australia
5 Australian Research Council (ARC), Centre of Excellence in Advanced Molecular Imaging, Australia
6 Istituto Italiano di Tecnologia, Genova 16163, Italy
7 School of Physics, University of Melbourne, Victoria 3010, Australia
8 School of Engineering, RMIT University, Melbourne, Victoria 3001, Australia
9 Microvision and Microdiagnostic Group (SCI STI CHD), Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland
10 Laboratory for Cellular Imaging and Energetics, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia
11 Joint International Research Unit in Neurodevelopment and Child Psychiatry, CHUV, Département de Psychiatrie, Lausanne, Switzerland, Université Laval, Québec, Canada
12 Institut universitaire en santé mentale de Québec, Québec, Canada
13 Centre d’optique, photonique et laser, Department of Psychiatry and Neuroscience, Université Laval, 2375 rue de la Terrasse, Québec, QC G1V 0A6, Canada
14 e-mail: xcyuan@szu.edu.cn
15 e-mail: christian.depeursinge@epfl.ch
16 e-mail: Pierre.Marquet@neuro.ulaval.ca
17 e-mail: s.kou@latrobe.edu.au
Abstract
We report a dual-contrast method of simultaneously measuring and visualizing the volumetric structural information in live biological samples in three-dimensional (3D) space. By introducing a direct way of deriving the 3D scattering potential of the object from the synthesized angular spectra, we obtain the quantitative subcellular morphology in refractive indices (RIs) side-by-side with its fluorescence signals. The additional contrast in RI complements the fluorescent signal, providing additional information of the targeted zones. The simultaneous dual-contrast 3D mechanism unveiled interesting information inaccessible with previous methods, as we demonstrated in the human immune cell (T cell) experiment. Further validation has been demonstrated using a Monte Carlo model.

Chen Liu, Michael Malek, Ivan Poon, Lanzhou Jiang, Arif M. Siddiquee, Colin J. R. Sheppard, Ann Roberts, Harry Quiney, Douguo Zhang, Xiaocong Yuan, Jiao Lin, Christian Depeursinge, Pierre Marquet, Shan Shan Kou. Simultaneous dual-contrast three-dimensional imaging in live cells via optical diffraction tomography and fluorescence[J]. Photonics Research, 2019, 7(9): 09001042.

本文已被 2 篇论文引用
被引统计数据来源于中国光学期刊网
引用该论文: TXT   |   EndNote

相关论文

加载中...

关于本站 Cookie 的使用提示

中国光学期刊网使用基于 cookie 的技术来更好地为您提供各项服务,点击此处了解我们的隐私策略。 如您需继续使用本网站,请您授权我们使用本地 cookie 来保存部分信息。
全站搜索
您最值得信赖的光电行业旗舰网络服务平台!