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基于角度照明优化的傅里叶叠层显微成像方法

An Efficient Fourier Ptychographic Microscopy Imaging Method Based on Angle Illumination Optimization

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摘要

提出一种基于LED角度照明优化模式的傅里叶叠层显微成像方法。首先,根据LED、孔径和样品之间的关系,获取傅里叶平面可扩展的理论频谱范围;其次,使用图像质量评价指标衡量不同照明方式下的重建图像质量差异,构造任意单个LED对整体重建结果影响的差异函数;然后,通过对差异表达式的分析与仿真,制定了最佳的角度照明策略,设计出一种基于菱形采样方法来加速傅里叶叠层显微成像的实现过程;最后,采用主观和客观的评价指标对仿真和实验的有效性进行评估。结果表明,本文方法在保持重建质量的前提下有效提高了成像效率,其效率可提高到传统方法的3.85倍。

Abstract

In this study, an efficient Fourier ptychographic microscopy method (FPM) based on an optimized pattern of light emitting diode (LED) angle illumination is proposed. First, a theoretical expandable spectrum range is obtained in the Fourier space based on the relationship among the LED, the aperture, and the sample. Second, image quality assessment methods are utilized to extract differential expressions to describe how an arbitrary LED affects the reconstruction effect. Third, the optimized angle illumination strategy is implemented based on the analysis of the differential expression. Then, a rhombus-based sampling approach is designed to accelerate the FPM process. Finally, the subjective and objective assessments are used to measure the validity of both simulation and actual experiments. Results indicate that our optimized method can effectively improve the efficiency by approximately 3.85 times compared with that of the traditional method.

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中图分类号:O436

DOI:10.3788/LOP57.081106

所属栏目:成像系统

基金项目:国家自然科学基金、浙江省自然科学基金、浙江省医药卫生科技项目、浙江省科协“育才工程”项目;

收稿日期:2019-10-21

修改稿日期:2019-11-19

网络出版日期:2020-04-01

作者单位    点击查看

李通:杭州电子科技大学电子信息学院, 浙江 杭州 310018
赵巨峰:杭州电子科技大学电子信息学院, 浙江 杭州 310018
毛海锋:杭州电子科技大学电子信息学院, 浙江 杭州 310018
崔光茫:杭州电子科技大学电子信息学院, 浙江 杭州 310018
胡金星:中国科学院大学宁波华美医院, 浙江 宁波 315010

联系人作者:赵巨峰(dabaozjf@hdu.edu.cn)

备注:国家自然科学基金、浙江省自然科学基金、浙江省医药卫生科技项目、浙江省科协“育才工程”项目;

【1】Ou X Z, Horstmeyer R, Yang C, et al. Quantitative phase imaging via Fourier ptychographic microscopy [J]. Optics Letters. 2013, 38(22): 4845-4848.

【2】Zheng G A, Horstmeyer R, Yang C. Wide-field, high-resolution Fourier ptychographic microscopy [J]. Nature Photonics. 2013, 7(9): 739-745.

【3】Sun J S, Zhang Y Z, Chen Q, et al. Fourier ptychographic microscopy: theory, advances, and applications [J]. Acta Optica Sinica. 2016, 36(10): 1011005.
孙佳嵩, 张玉珍, 陈钱, 等. 傅里叶叠层显微成像技术: 理论、发展和应用 [J]. 光学学报. 2016, 36(10): 1011005.

【4】Fienup J R. Phase retrieval algorithms: a personal tour [J]. Applied Optics. 2013, 52(1): 45-56.

【5】Song P M, Jiang S W, Zhang H, et al. Super-resolution microscopy via ptychographic structured modulation of a diffuser [J]. Optics Letters. 2019, 44(15): 3645-3648.

【6】Jiang S W, Liao J, Bian Z C, et al. Axially shifted pattern illumination for macroscale turbidity suppression and virtual volumetric confocal imaging without axial scanning [J]. Optics Letters. 2019, 44(4): 811-814.

【7】Li S F, Zhao Y, Chen G H, et al. Extraction of particle size via Fourier ptychography with selective illuminations [J]. Infrared and Laser Engineering. 2017, 46(11): 1103005.
李生福, 赵宇, 陈光华, 等. 选择型照明傅里叶叠层成像提取粒子尺度 [J]. 红外与激光工程. 2017, 46(11): 1103005.

【8】Lin Z Q, Ma X, Lin J X, et al. Fourier ptychographic microscopy based on rotatingarc-shaped array of LEDs [J]. Laser & Optoelectronics Progress. 2018, 55(7): 071102.
林子强, 马骁, 林锦新, 等. 基于弧形阵列LED光源旋转照明装置的傅里叶叠层显微术 [J]. 激光与光电子学进展. 2018, 55(7): 071102.

【9】Yang J Q, Ma X, Lin J X, et al. Intensity correction research for Fourier ptychographic microscopy [J]. Laser & Optoelectronics Progress. 2017, 54(3): 031101.
杨佳琪, 马骁, 林锦新, 等. 傅里叶叠层显微术的照明光强校正研究 [J]. 激光与光电子学进展. 2017, 54(3): 031101.

【10】Kuang C F, Ma Y, Zhou R J, et al. Digital micromirror device-based laser-illumination Fourier ptychographic microscopy [J]. Optics Express. 2015, 23(21): 26999-27010.

【11】Zhou A, Chen N, Wang H C, et al. Analysis of Fourier ptychographic microscopy with half of the captured images [J]. Journal of Optics. 2018, 20(9): 095701.

【12】Dong S Y, Shiradkar R, Nanda P, et al. Spectral multiplexing and coherent-state decomposition in Fourier ptychographic imaging [J]. Biomedical Optics Express. 2014, 5(6): 1757-1767.

【13】Zhou Y, Wu J M, Bian Z C, et al. Fourier ptychographic microscopy using wavelength multiplexing [J]. Journal of Biomedical Optics. 2017, 22(6): 066006.

【14】Guo K K, Dong S Y, Nanda P, et al. Optimization of sampling pattern and the design of Fourier ptychographic illuminator [J]. Optics Express. 2015, 23(5): 6171-6180.

【15】Zhang Y B, Jiang W X, Tian L, et al. Self-learning based Fourier ptychographic microscopy [J]. Optics Express. 2015, 23(14): 18471-18486.

【16】Bian L H, Suo J L, Situ G H, et al. Content adaptive illumination for Fourier ptychography [J]. Optics Letters. 2014, 39(23): 6648-6651.

【17】Bian L H, Suo J L, Zheng G A, et al. Fourier ptychographic reconstruction using Wirtinger flow optimization [J]. Optics Express. 2015, 23(4): 4856-4866.

【18】Zuo C, Sun J S, Chen Q. Adaptive step-size strategy for noise-robust Fourier ptychographic microscopy [J]. Optics Express. 2016, 24(18): 20724-20744.

【19】Jiang S W, Guo K K, Liao J, et al. Solving Fourier ptychographic imaging problems via neural network modeling and TensorFlow [J]. Biomedical Optics Express. 2018, 9(7): 3306.

【20】Wang Z, Bovik A C, Sheikh H R, et al. Image quality assessment: from error visibility to structural similarity [J]. IEEE Transactions on Image Processing. 2004, 13(4): 600-612.

引用该论文

Li Tong,Zhao Jufeng,Mao Haifeng,Cui Guangmang,Hu Jinxing. An Efficient Fourier Ptychographic Microscopy Imaging Method Based on Angle Illumination Optimization[J]. Laser & Optoelectronics Progress, 2020, 57(8): 081106

李通,赵巨峰,毛海锋,崔光茫,胡金星. 基于角度照明优化的傅里叶叠层显微成像方法[J]. 激光与光电子学进展, 2020, 57(8): 081106

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