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基于光学相干层析成像的皮层血流高分辨率成像

High-Resolution Cortical Blood Flow Imaging Based on Optical Coherence Tomography

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摘要

光学相干层析成像(OCT)技术具有非接触、分辨率高、采集速度快等优势,不仅能够显示样品的三维结构,而且能够检测样品中的运动信息。构建了高分辨率光谱OCT血管成像系统,系统横向分辨率约为6.7 μm,纵向分辨率约为4.7 μm,相机线采集速度140 kHz,能够在2 s内完成三维扫描。在此基础上,通过相邻B扫描图像差分运算提取血流信息,实现血管成像。应用该系统扫描了大鼠大脑皮层的三维血管网络,实验结果表明,系统具有对毛细血管成像的能力。

Abstract

Optical coherence tomography (OCT) is a non-contact imaging modality with advantages of high resolution and high acquisition speed. OCT can provide three-dimensional image structures and detect motion information in the samples. In this study, a high-resolution spectral OCT angiography system was constructed with the lateral and axial resolutions of about 6.7 μm and about 4.7 μm, respectively. The line scan camera was operated at 140 kHz and could complete a three-dimensional scan within 2 s. Based on this, the blood flow perfusion information was extracted by differentiating the adjacent B-scan images. A micro-vasculature network image of rat cerebral cortex was obtained. The experimental results demonstrate that the proposed system is suitable for capillary imaging.

Newport宣传-MKS新实验室计划
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DOI:10.3788/LOP56.111101

所属栏目:成像系统

基金项目:国家自然科学基金、中央高校基本科研业务费、河北省自然科学基金;

收稿日期:2018-10-12

修改稿日期:2019-01-10

网络出版日期:2019-06-13

作者单位    点击查看

高英哲:燕山大学电气工程学院, 河北 秦皇岛 066004
袁毅:燕山大学电气工程学院, 河北 秦皇岛 066004
马振鹤:东北大学秦皇岛分校控制工程学院, 河北 秦皇岛 066004

联系人作者:高英哲, 袁毅, 马振鹤(617226922@qq.com, yuanyi513@163.com, mazhenhe@163.com)

备注:国家自然科学基金、中央高校基本科研业务费、河北省自然科学基金;

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引用该论文

Yingzhe Gao, Yi Yuan, Zhenhe Ma. High-Resolution Cortical Blood Flow Imaging Based on Optical Coherence Tomography[J]. Laser & Optoelectronics Progress, 2019, 56(11): 111101

高英哲, 袁毅, 马振鹤. 基于光学相干层析成像的皮层血流高分辨率成像[J]. 激光与光电子学进展, 2019, 56(11): 111101

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