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氧化石墨烯修饰腐蚀型长周期光纤光栅的禽流感病毒免疫传感器

Avian Influenza Virus Immunosensor Based on Etched Long Period Fiber Grating Coated with Graphene Oxide

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摘要

提出了一种使用基于氧化石墨烯修饰包层腐蚀型长周期光纤光栅应用于检测禽流感病毒的免疫传感器.氧化石墨烯通过氢键结合在包层腐蚀型长周期光纤光栅表面上,并通过共价键将禽流感病毒单克隆抗体与氧化石墨烯表面的羧基相结合.利用氧化石墨烯上吸附的禽流感病毒单克隆抗体与禽流感病毒抗原的特异性结合引起的长周期光纤光栅谐振波长变化进行检测.结果表明,该氧化石墨烯修饰包层腐蚀型长周期光纤光栅免疫传感器对禽流感病毒的检测极限为40 ng/mL,传感器的解离常数为~1.6×10-7 mol/L,检测范围为40 ng/mL~200 μg/mL.通过对禽流感病毒空白尿囊液、禽流感病毒尿囊液和新城疫病毒尿囊液进行检测,表明免疫传感器具有良好的特异性和临床性.该免疫传感器具有应用于禽流感病毒的快速和早期诊断的可能.

Abstract

An immunosensor based on graphene oxide modified cladding etched long-period fiber grating sensor for detection of avian influenza virus is presented in this paper. Graphene oxide is coated on the surface of long-period fiber grating by hydrogen bonding using sodium hydroxide and the avian influenza virus monoclonal antibodies are combined with graphene oxide surface by a covalent bond. The resonant wavelength change of long-period fiber grating caused by the specific binding of avian influenza virus monoclonal antibody aolsorbed on graphene oxide to avian influenza virus antigen was used for detection. The experimental results show that the limit of detection of the graphene oxide modified cladding etched long-period fiber grating immunesensor can reach 40 ng/mL, the dissociation constant ~1.6×10-7 M and the detection range 40 ng/mL~200μg/mL. The detection results of avian influenza virus in avian influenza virus blank allantoic fluid, Avian influenza virus allantoic fluid and Newcastle disease virus allantoic fluid show that the immunosensor has good specificity and clinical characteristics. Therefore, the immunosensor could be potentially applied in the fast detection and early diagnosis of avian influenza virus.

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中图分类号:TN253

DOI:10.3788/gzxb20204901.0106002

所属栏目:光纤光学与光通信

基金项目:|National Natural Science Foundation of China (Nos.61875026, 61505017), the Foundation and Cutting-edge Research Projects of Chongqing Science and Technology Commission (Nos.cstc 2019jcyj-msxmX0093, cstc2018jcyjAX0122), the Youth Project of Science and Technology Research Program of Chongqing Education Commission of China (No.KJQN201801121), Graduate Student Innovation Program of Chongqing University of Technology (Nos.ycx2018235, ycx2018231)

收稿日期:2019-07-25

修改稿日期:2019-09-18

网络出版日期:2020-03-19

作者单位    点击查看

石胜辉:重庆理工大学 光纤传感与光电检测重庆市重点实验室, 重庆 400054
王鑫:重庆理工大学 光纤传感与光电检测重庆市重点实验室, 重庆 400054
罗彬彬:重庆理工大学 光纤传感与光电检测重庆市重点实验室, 重庆 400054
王亚杰:重庆理工大学 光纤传感与光电检测重庆市重点实验室, 重庆 400054
刘志江:重庆理工大学 光纤传感与光电检测重庆市重点实验室, 重庆 400054
聂青林:重庆理工大学 光纤传感与光电检测重庆市重点实验室, 重庆 400054
马培杰:重庆理工大学 药物化学与分子药理学重庆市重点实验室, 重庆 400054
吕清明:重庆理工大学 光纤传感与光电检测重庆市重点实验室, 重庆 400054
鲁友铭:重庆理工大学 药物化学与分子药理学重庆市重点实验室, 重庆 400054
吴胜昔:重庆理工大学 药物化学与分子药理学重庆市重点实验室, 重庆 400054
赵明富:重庆理工大学 光纤传感与光电检测重庆市重点实验室, 重庆 400054
巫涛江:重庆能源职业学院, 重庆 402247

联系人作者:罗彬彬(luobinbin@cqut.edu.cn)

备注:|National Natural Science Foundation of China (Nos.61875026, 61505017), the Foundation and Cutting-edge Research Projects of Chongqing Science and Technology Commission (Nos.cstc 2019jcyj-msxmX0093, cstc2018jcyjAX0122), the Youth Project of Science and Technology Research Program of Chongqing Education Commission of China (No.KJQN201801121), Graduate Student Innovation Program of Chongqing University of Technology (Nos.ycx2018235, ycx2018231)

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引用该论文

Sheng-hui SHI,Xin WANG,Bin-bin LUO,Ya-jie WANG,Zhi-jiang LIU,Qing-lin NIE,Pei-jie MA,Qing-ming Lü,You-ming LU,Sheng-xi WU,Ming-fu ZHAO,Tao-jiang WU. Avian Influenza Virus Immunosensor Based on Etched Long Period Fiber Grating Coated with Graphene Oxide[J]. ACTA PHOTONICA SINICA, 2020, 49(1): 0106002

石胜辉,王鑫,罗彬彬,王亚杰,刘志江,聂青林,马培杰,吕清明,鲁友铭,吴胜昔,赵明富,巫涛江. 氧化石墨烯修饰腐蚀型长周期光纤光栅的禽流感病毒免疫传感器[J]. 光子学报, 2020, 49(1): 0106002

被引情况

【1】石胜辉,吴德操,王鑫,聂青林,刘志江,罗彬彬,刘恩华,柳鹏,赵明富. 氧化石墨烯包覆金纳米壳修饰长周期光栅的免疫传感器. 光学学报, 2020, 40(18): 1806001--1

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