A novel type of mid-IR microresonator, the chalcogenide glass (ChG) microfiber knot resonator (MKR), is demonstrated, showing easy fabrication, fiber-compatible features, resonance tunability, and high robustness. ChG microfibers with typical diameters around 3 μm are taper-drawn from As2S3 glass fibers and assembled into MKRs in liquid without surface damage. The measured Q factor of a typical 824 μm diameter ChG MKR is about 2.84×104 at the wavelength of 4469.14 nm. The free spectral range (FSR) of the MKR can be tuned from 2.0 nm (28.4 GHz) to 9.6 nm (135.9 GHz) by tightening the knot structure in liquid. Benefitting from the high thermal expansion coefficient of As2S3 glass, the MKR exhibits a thermal tuning rate of 110pm·°C?1 at the resonance peak. When embedded in polymethyl methacrylate (PMMA) film, a 551 μm diameter MKR retains a Q factor of 1.1×104. The ChG MKRs demonstrated here are highly promising for resonator-based optical technologies and applications in the mid-IR spectral range.