中国激光, 2020, 47 (2): 0207005, 网络出版: 2020-02-21
相干拉曼散射显微技术及其在生物医学领域的应用 下载: 3067次特邀综述
Coherent Raman Scattering Microscopy Technique and Its Biomedical Applications
生物医学 相干拉曼散射显微技术 相干反斯托克斯拉曼散射 无标记成像 受激拉曼散射成像 biomedicine coherent Raman scattering microscopy technique coherent anti-stokes Raman scattering label-free imaging stimulated Raman scattering imaging
摘要
相干拉曼散射显微技术作为一种新型的成像技术,具有无标记、高特异性、非侵入等优点,已被广泛用于化学结构及物质成分分析。近年来,光子学、生物医学和显微成像技术等领域的相互交叉和融合发展,极大地推动了相干拉曼散射显微成像技术在生物医学领域的应用。简要介绍了相干拉曼散射显微成像的基本原理、分类、系统构成,同时概述了相干拉曼散射显微成像技术近年来在生物医学领域的应用,包括检测、脂类分析和蛋白质构象变化等,最后对其未来发展进行了展望。
Abstract
As a new imaging technique, coherent Raman scattering (CRS) microscopy has been widely used in chemical structure and composition analysis because of its advantages of label-free, high specificity, and on-invasion. In recent years, the mutual cross-over and integrated development of photonics, biomedicine, and microscopic imaging technology has greatly promoted the application of CRS microscopy in biomedicine. This paper briefly introduces the basic principle of CRS microscopy and its classification, then explains the most widely used implementation of CRS, and summarizes the recent applications of CRS microscopic imaging in biomedicine, including detection, lipid analysis, and protein conformation change. Finally, the future development of CRS is discussed.
李姿霖, 李少伟, 张思鹭, 沈炳林, 屈军乐, 刘丽炜. 相干拉曼散射显微技术及其在生物医学领域的应用[J]. 中国激光, 2020, 47(2): 0207005. Li Zilin, Li Shaowei, Zhang Silu, Shen Binglin, Qu Junle, Liu Liwei. Coherent Raman Scattering Microscopy Technique and Its Biomedical Applications[J]. Chinese Journal of Lasers, 2020, 47(2): 0207005.